Actin Antikörper (N-Term)

Produktnummer ABIN5540760

Der Maus Monoklonal Anti-Actin-Antikörper wurde für WB, IHC (p), IHC (fro) und EIA validiert. Er ist geeignet, Actin in Proben von Human, Ratte, Kaninchen und Schwein zu detektieren.

Kurzübersicht für Actin Antikörper (N-Term) (ABIN5540760)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reaktivität: Human, Ratte, Kaninchen, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 3B3

Produktdetails anti-Actin Antikörper

Bindungsspezifität: N-Term

Sequenz: Ac-DEDETTALVC -COOH

Spezifität: This 3B3 Monoclonal antibody is highly specific for Alpha-Skeletal Actin, and does not cross react with other Actin isoforms.

Aufreinigung: Purified

Immunogen: Peptide comprising the N-terminal nonapeptide of alpha-skeletal Actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin through the cysteine residue.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Flow Cytometry. Immunoblotting. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Sections. Recommended Dilutions : 1/100-1/1000 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/1000-1/5000 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Andere Bezeichnung: alpha skeletal muscle actin,acta1

Hintergrund: Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. Skeletal α-actin may also be expressed in the mammalian heart. Although during the late stage of fetal development α-cardiac actin becomes the main isoform in the myocardium, α-skeletal actin might still make up for almost half of its total striated muscle actin content.

Pathways: Caspase Kaskade in der Apoptose, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development