Actin Antikörper

Produktnummer ABIN5540756

Dieses Huhn Polyklonal-Antikörper erkennt spezifisch Actin in WB. Er zeigt eine Reaktivität gegenüber Kaninchen.

Kurzübersicht für Actin Antikörper (ABIN5540756)

Target: Actin

Reaktivität: Kaninchen

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Actin Antikörper

Aufreinigung: Affinity Chromatography

Immunogen: Chickens were immunized with actin purified from Rabbit muscle. After epeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. The final product contains 50% glycerol (v/v), purified anti-Actin IgY fraction (10 mg/ml) spiked with 25 mug/ml of affinity purified anti-Actin IgY, and then filter-sterilized.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Quality Control: This anti-Actin preparation was validated by Western blotting on nitrocellulose membranes (at a concentration of 1 μg/mL) using HRP-Conjugated Goat anti-Chicken IgY (1/5000 dilution, Cat.-No AP31795HR-N) as the secondary reagent. Recommended Dilutions: 1/1000-1/2000 for Western blot analysis of Nitrocellulose membranes. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary. Do Not use this product with PVDF, or other nylon-based membranes, because this often produces unacceptably high backgrounds in chemiluminescence studies.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C This product is photosensitive and should be protected from light. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Actin

Target: Actin