GAP43 Antikörper (C-Term)

Produktnummer ABIN5540033

Dieses Huhn Polyklonal-Antikörper erkennt spezifisch GAP43 in WB, IF, ICC und IHC (p). Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für GAP43 Antikörper (C-Term) (ABIN5540033)

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Reaktivität: Maus

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser GAP43 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GAP43 Antikörper

Bindungsspezifität: C-Term

Spezifität: Specific for the ~43k GAP-43 protein.

Aufreinigung: Affinity Chromatography

Immunogen: Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of Mouse GAP-43 coupled to keyhole limpet hemocyanin. Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1/10 with PBS containing BSA as a carrier. Finally, the antibody preparation was filter-sterilized.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Quality Control: Quality assurance analysis was performed using Immunohistochemistry (at 1/1000 dilution) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/1000 dilution) as a secondary antibody. Recommended Dilutions: 1/1000-1/2000 for Immunohistochemstry and Immunocytochemistry using 2 % paraformaldehyde-fixed tissues or cells and 1/2000-1/5000 for western blots.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.2 Preservatives: 0.02 % Sodium Azide Stabilizers: 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 2-8°C. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Andere Bezeichnung: gap43

Hintergrund: GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). GAP-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C modulates the interaction of GAP-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).

UniProt: P17677