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Intestinal Alkaline Phosphatase Antikörper (HRP)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Intestinal Alkaline Phosphatase in WB und EIA. Er zeigt eine Reaktivität gegenüber Rind (Kuh).
Produktnummer ABIN5539994

Kurzübersicht für Intestinal Alkaline Phosphatase Antikörper (HRP) (ABIN5539994)

Target

Alle Intestinal Alkaline Phosphatase (ALPI) Antikörper anzeigen
Intestinal Alkaline Phosphatase (ALPI)

Reaktivität

  • 46
  • 21
  • 4
  • 3
Rind (Kuh)

Wirt

  • 47
  • 12
  • 6
  • 5
Kaninchen

Klonalität

  • 57
  • 13
Polyklonal

Konjugat

  • 44
  • 8
  • 6
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Intestinal Alkaline Phosphatase Antikörper ist konjugiert mit HRP

Applikation

  • 55
  • 38
  • 28
  • 13
  • 8
  • 6
  • 5
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)
  • Spezifität

    This product detects bovine Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Alkaline phosphatase [calf intestine].

    Aufreinigung

    Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

    Immunogen

    Alkaline phosphatase from calf intestine
  • Applikationshinweise

    Western Blot : 1/200-1/1,000. ELISA : 1/2,000-1/10,000. Anti Alkaline phosphatase has been assayed against 1.0 μg of Alkaline phosphatase [E.coli] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,500 to 1/15,000 of the reconstitution concentration is suggested.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

    Haltbarkeit

    12 months
  • Target

    Intestinal Alkaline Phosphatase (ALPI)

    Andere Bezeichnung

    alkaline phosphatase,alpi,iap

    UniProt

    P19111
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