Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Intestinal Alkaline Phosphatase in WB und EIA. Er zeigt eine Reaktivität gegenüber Rind (Kuh).
Produktnummer ABIN5539994
Kurzübersicht für Intestinal Alkaline Phosphatase Antikörper (HRP) (ABIN5539994)
This product detects bovine Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Alkaline phosphatase [calf intestine].
Aufreinigung
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Western Blot : 1/200-1/1,000. ELISA : 1/2,000-1/10,000. Anti Alkaline phosphatase has been assayed against 1.0 μg of Alkaline phosphatase [E.coli] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,500 to 1/15,000 of the reconstitution concentration is suggested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.