Intestinal Alkaline Phosphatase Antikörper
Kurzübersicht für Intestinal Alkaline Phosphatase Antikörper (ABIN5539990)
Target
Alle Intestinal Alkaline Phosphatase (ALPI) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Spezifität
- This antibody detects bovine alkaline phosphatase. Cross reactivity against alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-Goat Serum as well as purified and partially purified alkaline phosphatase [calf intestine]. Species: Bovine.
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Aufreinigung
- Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
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Immunogen
- Alkaline phosphatase from calf intestine
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Isotyp
- IgG
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Applikationshinweise
- ELISA : 1/4000-1/20000. Western Blot : 1/500-1/2000. Immunoblotting (Western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Buffer
- 0.01M Sodium phosphate, 0.14M Sodium chloride, pH 7.4
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Haltbarkeit
- 12 months
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- Intestinal Alkaline Phosphatase (ALPI)
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Andere Bezeichnung
- alkaline phosphatase,alpi,iap
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Hintergrund
- Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
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UniProt
- P19111
Target
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