Intestinal Alkaline Phosphatase Antikörper

Produktnummer ABIN5539990

Dieses Anti-Intestinal Alkaline Phosphatase-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Intestinal Alkaline Phosphatase in WB, IP, EIA und DB. Geeignet für Rind (Kuh).

Kurzübersicht für Intestinal Alkaline Phosphatase Antikörper (ABIN5539990)

Target: Intestinal Alkaline Phosphatase (ALPI)

Reaktivität: Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Intestinal Alkaline Phosphatase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB)

Produktdetails anti-Intestinal Alkaline Phosphatase Antikörper

Spezifität: This antibody detects bovine alkaline phosphatase. Cross reactivity against alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-Goat Serum as well as purified and partially purified alkaline phosphatase [calf intestine]. Species: Bovine.

Aufreinigung: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Alkaline phosphatase from calf intestine

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA : 1/4000-1/20000. Western Blot : 1/500-1/2000. Immunoblotting (Western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.01M Sodium phosphate, 0.14M Sodium chloride, pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Andere Bezeichnung: alkaline phosphatase,alpi,iap

Hintergrund: Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

UniProt: P19111