ESR2 Antikörper (AA 417-428)

Produktnummer ABIN5539801

Dieses Anti-ESR2-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von ESR2 in WB, ELISA und IF. Geeignet für Human.

Kurzübersicht für ESR2 Antikörper (AA 417-428) (ABIN5539801)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ESR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-ESR2 Antikörper

Bindungsspezifität: AA 417-428

Verwendungszweck: Estrogen receptor beta (aa417-428)

Sequenz: TQDADSSRKL AH

Spezifität: This antibody is expected to recognize all reported isoforms (NP_001428.1, NP_001035365.1, NP_001201831.1, NP_001201832.1). Reported variants represent identical protein: NP_001035365.1, NP_001035366.1

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 50 kDa band observed in Human Placenta lysates (calculated MW of 53.0 kDa according to NP_001035366.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nucleus of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Andere Bezeichnung: ESR2

Hintergrund: ESR2, estrogen receptor 2 (ER beta), ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, OTTHUMP00000180647, estrogen nuclear receptor beta variant a, estrogen nuclear receptor beta variant b, estrogen receptor beta, estrogen receptor beta 4, nuclear receptor sub

Gen-ID: 2100

NCBI Accession: NP_001428, NP_001035365, NP_001201831, NP_001201832

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling