HDAC1 Antikörper (C-Term)

Produktnummer ABIN5539534

Dieses Anti-HDAC1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von HDAC1 in WB, ELISA, IF und ChIP. Geeignet für Human und Maus.

Kurzübersicht für HDAC1 Antikörper (C-Term) (ABIN5539534)

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HDAC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Güteklasse: Verified

Produktdetails anti-HDAC1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Histone Deacetylase 1

Sequenz: KPEAKGVKEE VK

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Appox 55 kDa band observed in Human Duodenum and Lymph Node lysates and approx 70 kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 55.1 kDa according to Human NP_004955.2 and Mouse NP_032254.1). The observed 70 kDa

Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10μg/ml

Chromatin Immunoprecipitation: A 3.5-fold increased presence of H3 onto the Human P21 l

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Andere Bezeichnung: HDAC1

Hintergrund: HDAC1, histone deacetylase 1, DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1 , reduced potassium dependency, yeast homolog-like 1

Gen-ID: 3065

NCBI Accession: NP_004955

Pathways: Neurotrophin Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis