GCAP1 Antikörper (C-Term)

Produktnummer ABIN5531246

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GCAP1 in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für GCAP1 Antikörper (C-Term) (ABIN5531246)

Target: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GCAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GCAP1 Antikörper

Bindungsspezifität: AA 160-191, C-Term

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogen: This GCAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-191 amino acids from the C-terminal region of human GCAP1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu GCAP1

Target: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Andere Bezeichnung: GCAP1

Hintergrund: Guanylate cyclase-activating protein is a l Ca(2+)-binding protein that upregulates synthesis of cGMP in photoreceptors. The known mammalian GCAPs are more than 90 % similar, consisting of 201 to 205 amino acids, and containing 3 identically conserved Ca(2+)-binding sites. The GUCA1A gene, also termed GCAP1, is transcribed into a single 1.7-kb mRNA species detectable only in the retina. In a 4-generation British family with typical clinical features of autosomal dominant cone dystrophy a tyr99-to-cys mutation) in the GUCA1A gene has been identified. Another family with a pro50-to-leu mutation in GUCA1A demonstrated phenotypic variability ranging from mild photophobia to rod-cone dystrophy. The mutant protein could activate guanylate cyclase 1 (GUCY2D) and displayed similar calcium sensitivity to wildtype protein. However, there was a marked increase in the susceptibility to protease degradation and a reduction in the thermal stability of the pro50-to-leu mutation, which may depress cellular concentration and thereby contribute to retinal cell mortality.

Molekulargewicht: 23 kDa

Gen-ID: 2978

UniProt: P43080

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction