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MED18 Antikörper (AA 1-208)

MED18 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC, FACS Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN5519036
  • Target Alle MED18 Antikörper anzeigen
    MED18 (Mediator Complex Subunit 18 (MED18))
    Bindungsspezifität
    • 15
    • 8
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-208
    Reaktivität
    • 52
    • 28
    • 20
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Human, Maus, Ratte
    Wirt
    • 51
    • 1
    Kaninchen
    Klonalität
    • 52
    Polyklonal
    Konjugat
    • 17
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser MED18 Antikörper ist unkonjugiert
    Applikation
    • 20
    • 13
    • 13
    • 13
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Verwendungszweck
    Anti-MED18 Antibody Picoband®
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins.
    Produktmerkmale
    Anti-MED18 Antibody Picoband® (ABIN5519036). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    E. coli-derived human MED18 recombinant protein (Position: M1-M208).
    Isotyp
    IgG
  • Applikationshinweise
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Hartz, P. A. Personal Communication. Baltimore, Md. 10/30/2008. 2. Sato, S., Tomomori-Sato, C., Banks, C. A. S., Sorokina, I., Parmely, T. J., Kong, S. E., Jin, J., Cai, Y., Lane, W. S., Brower, C. S., Conaway, R. C., Conaway, J. W. Identification of mammalian mediator subunits with similarities to yeast mediator subunits Srb5, Srb6, Med11, and Rox3. J. Biol. Chem. 278: 15123-15127, 2003.
    Kommentare

    We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target
    MED18 (Mediator Complex Subunit 18 (MED18))
    Andere Bezeichnung
    MED18 (MED18 Produkte)
    Hintergrund

    Synonyms: Mediator of RNA polymerase II transcription subunit 18, Mediator complex subunit 18, p28b, MED18,

    Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

    Background: MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II. Using in vitro-translated epitope-tagged proteins for protein-binding assays, it is found that MED18 ly interacted with the Mediator subunit TRFP (MED20). The MED18-TRFP heterodimer could also be coimmunoprecipitated from cotransfected insect cells. The MED18 gene was mapped to chromosome 1p35.3 based on an alignment of the MED18 sequence with the genomic sequence.

    Molekulargewicht
    24 kDa
    Gen-ID
    54797
    Pathways
    Regulation of Lipid Metabolism by PPARalpha
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