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MED18 Antikörper (AA 1-208)

Dieses Anti-MED18-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MED18 in WB, ELISA, IHC, IF, ICC und FACS. Geeignet für Human, Maus und Ratte.
Produktnummer ABIN5519036

Kurzübersicht für MED18 Antikörper (AA 1-208) (ABIN5519036)

Target

Alle MED18 Antikörper anzeigen
MED18 (Mediator Complex Subunit 18 (MED18))

Reaktivität

  • 55
  • 30
  • 22
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 54
  • 1
Kaninchen

Klonalität

  • 55
Polyklonal

Konjugat

  • 20
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser MED18 Antikörper ist unkonjugiert

Applikation

  • 23
  • 15
  • 13
  • 13
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 15
    • 8
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-208

    Verwendungszweck

    Anti-MED18 Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-MED18 Antibody Picoband® (ABIN5519036). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E. coli-derived human MED18 recombinant protein (Position: M1-M208).

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Hartz, P. A. Personal Communication. Baltimore, Md. 10/30/2008. 2. Sato, S., Tomomori-Sato, C., Banks, C. A. S., Sorokina, I., Parmely, T. J., Kong, S. E., Jin, J., Cai, Y., Lane, W. S., Brower, C. S., Conaway, R. C., Conaway, J. W. Identification of mammalian mediator subunits with similarities to yeast mediator subunits Srb5, Srb6, Med11, and Rox3. J. Biol. Chem. 278: 15123-15127, 2003.

    Kommentare

    We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    MED18 (Mediator Complex Subunit 18 (MED18))

    Andere Bezeichnung

    MED18

    Hintergrund

    Synonyms: Mediator of RNA polymerase II transcription subunit 18, Mediator complex subunit 18, p28b, MED18,

    Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

    Background: MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II. Using in vitro-translated epitope-tagged proteins for protein-binding assays, it is found that MED18 ly interacted with the Mediator subunit TRFP (MED20). The MED18-TRFP heterodimer could also be coimmunoprecipitated from cotransfected insect cells. The MED18 gene was mapped to chromosome 1p35.3 based on an alignment of the MED18 sequence with the genomic sequence.

    Molekulargewicht

    24 kDa

    Gen-ID

    54797

    Pathways

    Regulation of Lipid Metabolism by PPARalpha
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