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MED15 Antikörper (AA 1-285)

MED15 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, FACS, ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN5519035
  • Target Alle MED15 Antikörper anzeigen
    MED15 (Mediator Complex Subunit 15 (MED15))
    Bindungsspezifität
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-285
    Reaktivität
    • 30
    • 8
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Human, Maus, Ratte
    Wirt
    • 26
    • 5
    • 1
    Kaninchen
    Klonalität
    • 27
    • 5
    Polyklonal
    Konjugat
    • 22
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser MED15 Antikörper ist unkonjugiert
    Applikation
    • 20
    • 14
    • 7
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Verwendungszweck
    Anti-MED15 Antibody Picoband®
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins.
    Produktmerkmale
    Anti-MED15 Antibody Picoband® (ABIN5519035). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    E. coli-derived human MED15 recombinant protein (Position: M1-A285).
    Isotyp
    IgG
  • Applikationshinweise
    Western blot, 0.1-0.5 μg/mL, Human
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Fassler JS, Winston F (Dec 1989). "The Saccharomyces cerevisiae SPT13/GAL11 gene has both positive and negative regulatory roles in transcription". Molecular and Cellular Biology. 9 (12): 5602-9. 2. Suzuki Y, Nogi Y, Abe A, Fukasawa T (Nov 1988). "GAL11 protein, an auxiliary transcription activator for genes encoding galactose-metabolizing enzymes in Saccharomyces cerevisiae". Molecular and Cellular Biology. 8 (11): 4991-9.
    Kommentare

    We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target
    MED15 (Mediator Complex Subunit 15 (MED15))
    Andere Bezeichnung
    MED15 (MED15 Produkte)
    Hintergrund

    Synonyms: Mediator of RNA polymerase II transcription subunit 15, Activator-recruited cofactor 105 kDa component, ARC105, CTG repeat protein 7a, Mediator complex subunit 15, Positive cofactor 2 glutamine/Q-rich-associated protein, PC2 glutamine/Q-rich-associated protein, TPA-inducible gene 1 protein, TIG-1, Trinucleotide repeat-containing gene 7 protein, MED15, ARC105, CTG7A, PCQAP, TIG1, TNRC7,

    Tissue Specificity: Expressed in all tissues examined, including heart, brain, lung, spleen, thymus, pancreas, blood leukocyte and placenta. However, the level of expression varied, with highest expression in the placenta and peripheral blood and lowest in the pancreas and kidney.

    Background: Mediator of RNA polymerase II transcription subunit 15, also known as Gal11, Spt13 in yeast and PCQAP, ARC105, or TIG-1 in humans is a protein encoded by the MED15 gene. The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.

    Molekulargewicht
    97 kDa
    Gen-ID
    51586
    Pathways
    Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
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