MED9 Antikörper (AA 55-146)

Produktnummer ABIN5519032

Der Kaninchen Polyklonal Anti-MED9-Antikörper wurde für WB, ELISA, IF, FACS, ICC und IHC validiert. Er ist geeignet, MED9 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für MED9 Antikörper (AA 55-146) (ABIN5519032)

Target: MED9 (Mediator Complex Subunit 9 (MED9))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Produktdetails anti-MED9 Antikörper

Bindungsspezifität: AA 55-146

Verwendungszweck: Anti-MED9 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-MED9 Antibody Picoband® (ABIN5519032). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human MED9 recombinant protein (Position: A55-E146).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Tomomori-Sato, C., Sato, S., Parmely, T. J., Banks, C. A. S., Sorokina, I., Florens, L., Zybailov, B., Washburn, M. P., Brower, C. S., Conaway, R. C., Conaway, J. W. A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. J. Biol. Chem. 279: 5846-5851, 2004.

Kommentare:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu MED9

Target: MED9 (Mediator Complex Subunit 9 (MED9))

Andere Bezeichnung: MED9

Hintergrund:

Synonyms: Mediator of RNA polymerase II transcription subunit 9, Mediator complex subunit 9, MED9, MED25,

Tissue Specificity: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Background: MED9 is a member of the mediator complex of proteins that promote activation of RNA polymerase II through interactions with transcription factors. This MED9gene is located within the Smith-Magenis syndrome region on chromosome 17.

Molekulargewicht: 19 kDa

Gen-ID: 55090

Pathways: Regulation of Lipid Metabolism by PPARalpha