CCL20 Antikörper (AA 28-97)
Kurzübersicht für CCL20 Antikörper (AA 28-97) (ABIN5518973)
Target
Alle CCL20 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 28-97
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Verwendungszweck
- Anti-MIP-3 Alpha/CCL20 Antibody Picoband®
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Kreuzreaktivität (Details)
- No cross-reactivity with other proteins.
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Produktmerkmale
- Anti-MIP-3 Alpha/CCL20 Antibody Picoband® (ABIN5518973). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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Aufreinigung
- Immunogen affinity purified.
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Immunogen
- E. coli-derived mouse MIP-3 Alpha/CCL20 recombinant protein (Position: A28-M97).
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Isotyp
- IgG
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Applikationshinweise
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Western blot, 0.1-0.5 μg/mL
ELISA (Cap), 1-5 μg/mL
1. Harant, H., Eldershaw, S. A, Lindley, I. J. Human macrophage inflammatory protein-3-alpha/CCL20/LARC/EXODUS/SCYA20 is transcriptionally upregulated by tumor necrosis factor-alpha via a non-standard NF-kappa-B site. FEBS Lett. 509: 439-445, 2001. 2. Nelson, R. T., Boyd, J., Gladue, R. P., Paradis, T., Thomas, R., Cunningham, A. C., Lira, P., Brissette, W. H., Hayes, L., Hames, L. M., Neote, K. S., McColl, S. R. Genomic organization of the CC chemokine MIP-3-alpha/CCL20/LARC/EXODUS/SCYA20, showing gene structure, splice variants, and chromosome localization. Genomics 73: 28-37, 2001. -
Kommentare
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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Konzentration
- 500 μg/mL
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Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
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Andere Bezeichnung
- Ccl20
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Hintergrund
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Synonyms: C-C motif chemokine 20, Beta-chemokine exodus-1, CC chemokine LARC, CC chemokine ST38, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, Small-inducible cytokine A20, Ccl20, Larc, Scya20,
Tissue Specificity: Thymic medulla (at protein level). Prominently expressed in the small intestine, colon and appendix. Also found in thymus, spleen, lymph node and lung. The long form might be dominant in intestinal, and the short form in lymphoid tissues. Expressed by IL17 producing helper T-cells (Th17).
Background: Macrophage Inflammatory Protein 3alpha (MIP3alpha), also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4 (+) and CD8 (+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.
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Molekulargewicht
- 14 kDa
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Gen-ID
- 20297
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UniProt
- O89093
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Pathways
- Phosphorylierungen bei SARS-CoV-2 Infektion
Target
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