SLC6A1 Antikörper (N-Term)

Produktnummer ABIN5518957

Der Kaninchen Polyklonal Anti-SLC6A1-Antikörper wurde für WB validiert. Er ist geeignet, SLC6A1 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für SLC6A1 Antikörper (N-Term) (ABIN5518957)

Target: SLC6A1 (GAT1) (GABA Transporter 1 (GAT1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC6A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SLC6A1 Antikörper

Bindungsspezifität: AA 23-54, N-Term

Verwendungszweck: Anti-GABA Transporter 1/GAT 1/SLC6A1 Antibody Picoband®

Sequenz: ANDKPKTLVV KVQKKAADLP DRDTWKGRFD FL

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-GABA Transporter 1/GAT 1/SLC6A1 Antibody Picoband® (ABIN5518957). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SLC6A1, different from the related mouse and rat sequences by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Carvill, G. L., McMahon, J. M., Schneider, A., Zemel, M., Myers, C. T., Saykally, J., Nguyen, J., Robbiano, A., Zara, F., Specchio, N., Mecarelli, O., Smith, R. L., and 13 others. Mutations in the GABA transporter SLC6A1 cause epilepsy with myoclonic-atonic seizures. Am. J. Hum. Genet. 96: 808-815, 2015. Huang, F., Shi, L. J., 2. Cope, D. W., Di Giovanni, G., Fyson, S. J., Orban, G., Errington, A. C., Lorincz, M. L., Gould, T. M., Carter, D. A., Crunelli, V. Enhanced tonic GABA-A inhibition in typical absence epilepsy. Nature Med. 15: 1392-1398, 2009. 3. Heng, H. H. Q., Fei, J., Guo, L.-H. Assignment of the human GABA transporter gene (GABATHG) locus to chromosome 3p24-p25. Genomics 29: 302-304, 1995.

Kommentare:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu SLC6A1

Target: SLC6A1 (GAT1) (GABA Transporter 1 (GAT1))

Andere Bezeichnung: SLC6A1

Hintergrund:

Synonyms: Sodium- and chloride-dependent GABA transporter 1,GAT-1,Solute carrier family 6 member 1,SLC6A1,GABATR, GABT1, GAT1,

Tissue Specificity: Expressed in many tissues, highest levels in skeletal muscle.

Background: GABA transporter 1 (GAT1), also known as sodium- and chloride-dependent GABA transporter 1, is a protein that in humans is encoded by the SLC6A1 gene. GABA Transporter 1 uses Na+ and Cl- to create a gradient, which removes or adds GABA to extracellular spaces in the cerebrum and cerebellum. The stoichiometry for GABA Transporter 1 is 2 Na+: 1 Cl-: 1 GABA. The activity of GAT1 is largely dependent on the presence of Na+, while Cl- assists by increasing the ability for GAT-1 to uptake GABA.

Molekulargewicht: 88 kDa

Gen-ID: 6529

UniProt: P30531