NOV Antikörper (C-Term)

Produktnummer ABIN5518938

Produktdetails anti-NOV Antikörper

Target: NOV (Nephroblastoma Overexpressed (NOV))

Bindungsspezifität: AA 334-357, C-Term

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOV Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: Anti-NOV/CCN3 Antibody Picoband®

Sequenz: HTNCPKNNEA FLQELELKTT RGKM

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NOV/CCN3 Antibody Picoband® (ABIN5518938). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NOV/CCN3, different from the related mouse sequence by seven amino acids, and from the related rat sequence by four amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Jun JI, Lau LF (Dec 2011). "Taking aim at the extracellular matrix: CCN proteins as emerging therapeutic targets". Nature Reviews. Drug Discovery. 10 (12): 945-63. 2. Martinerie C, Viegas-Pequignot E, Guenard I, Dutrillaux B, Nguyen VC, Bernheim A, Perbal B (Dec 1992). "Physical mapping of human loci homologous to the chicken nov proto-oncogene". Oncogene. 7 (12): 2529-34.

Kommentare:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu NOV

Target: NOV (Nephroblastoma Overexpressed (NOV))

Andere Bezeichnung: NOV (NOV Produkte)

Hintergrund:

Synonyms: Protein NOV homolog,NovH,CCN family member 3,Insulin-like growth factor-binding protein 9,IBP-9,IGF-binding protein 9,IGFBP-9,Nephroblastoma-overexpressed gene protein homolog,NOV,CCN3, IGFBP9, NOVH,

Tissue Specificity: Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. .

Background: NOV (nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development.

Molekulargewicht: 47 kDa

Gen-ID: 4856

UniProt: P48745

Pathways: Smooth Muscle Cell Migration, Growth Factor Binding