PPIA Antikörper (AA 116-165)
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- Target Alle PPIA Antikörper anzeigen
- PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))
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Bindungsspezifität
- AA 116-165
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser PPIA Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Verwendungszweck
- Anti-Cyclophilin A/PPIA Antibody Picoband®
- Kreuzreaktivität (Details)
- No cross-reactivity with other proteins
- Produktmerkmale
- Anti-Cyclophilin A/PPIA Antibody Picoband® (ABIN5518864). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Aufreinigung
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human Cyclophilin A recombinant protein (Position: T116-E165). Human Cyclophilin A shares 98% and 95.9% amino acid (aa) sequence identity with mouse and rat Cyclophilin A, respectively.
- Isotyp
- IgG
- Top Product
- Discover our top product PPIA Primärantikörper
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- Applikationshinweise
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Luban, J., Bossolt, K. L., Franke, E. K., Kalpana, G. V., Goff, S. P.Human immunodeficiency virus type 1 gag protein binds to Cyclophilin A and B.Cell 73: 1067-1078, 1993. 2. Manel, N., Hogstad, B., Wang, Y., Levy, D. E., Unutmaz, D., Littman, D. R.A cryptic sensor for HIV-1 activates antiviral innate immunity in dendritic cells.Nature 467: 214-217, 2010. Note: Erratum: Nature 470: 424 only, 2011. 3. Willenbrink, W., Halaschek, J., Schuffenhauer, S., Kunz, J., Steinkasserer, A. Cyclophilin A, the major intracellular receptor for the immunosuppressant cyclosporin A, maps to chromosome 7p11.2-p13: four pseudogenes map to chromosomes 3, 10, 14, and 18.Genomics 28: 101-104, 1995. - Kommentare
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Konzentration
- 500 μg/mL
- Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Target
- PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))
- Andere Bezeichnung
- PPIA (PPIA Produkte)
- Hintergrund
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Synonyms: Peptidyl-prolyl cis-trans isomerase A,PPIase A,5.2.1.8,Cyclophilin A,Cyclosporin A-binding protein,Rotamase A,Peptidyl-prolyl cis-trans isomerase A, N-terminally processed,PPIA,CYPA,
Tissue Specificity: Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
Background: Cyclophilin A (PPIA), Peptidylprolyl isomerase A, is an enzyme that in humans is encoded by the PPIA gene. Using chromosome 7 and chromosome 10 deletion hybrid panels, the PPIA coding gene is localized to 7p13-p11.2. This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. Cyclophilin A is also a member of the immunophilin class of proteins that all possess peptidyl-prolyl cis/trans isomerase activity and are believed to be involved in protein folding and/or intracellular protein transport. And Cyclophilin A binds to the Gag protein of human immunodeficiency virus type 1 (HIV-1). Additionally, Cyclophilin A may have an essential function in HIV-1 replication.may have an essential function in HIV-1 replication.
- Molekulargewicht
- 185 kDa
- Gen-ID
- 5478
- UniProt
- P62937
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