Nerve Growth Factor Antikörper (AA 122-241)

Produktnummer ABIN5518858

Der Kaninchen Polyklonal Anti-Nerve Growth Factor-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, Nerve Growth Factor in Proben von Human zu detektieren. Es sind 6+ Publikationen verfügbar.

Kurzübersicht für Nerve Growth Factor Antikörper (AA 122-241) (ABIN5518858)

Target: Nerve Growth Factor (NGF)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nerve Growth Factor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Nerve Growth Factor Antikörper

Bindungsspezifität: AA 122-241

Verwendungszweck: Anti-NGF/NGF Beta Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NGF/NGF Beta Antibody Picoband® (ABIN5518858). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human NGF/NGF Beta recombinant protein (Position: S122-A241). Human NGF/NGF Beta shares 92.4% amino acid (aa) sequence identity with rat NGF/NGF Beta.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Human
1. Dracopoli, N. C., Rose, E., Whitfield, G. K., Guidon, P. T., Bale, S. J., Chance, P. A., Kourides, I. A., Housman, D. E. : Two thyroid hormone regulated genes, the beta-subunits of nerve growth factor (NGFB) and thyroid stimulating hormone (TSHB), are located less than 310 kb apart in both human and mouse genomes. Genomics 3: 161-167, 1988. 2. Einarsdottir, E., Carlsson, A., Minde, J., Toolanen, G., Svensson, O., Solders, G., Holmgren, G., Holmberg, D., Holmberg, M. : A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception. Hum. Molec. Genet. 13: 799-805, 2004. 3. Francke, U., de Martinville, B., Coussens, L., Ullrich, A. : The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1. Science 222: 1248-1251, 1983

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Nerve Growth Factor Antikörper (ABIN5518858)

Fan, Xiong, Fu, Xu, Wang, Chen, Xia, Peng, Ye, Wang, Zhang, Ye et al.: "Polyaniline promotes peripheral nerve regeneration by enhancement of the brain‑derived neurotrophic factor and ciliary neurotrophic factor expression and activation of the ERK1/2/MAPK signaling ..." in: Molecular medicine reports, Vol. 16, Issue 5, pp. 7534-7540, (2018) (PubMed).

Zhao, Chen, Shen, Zhao, Wang, Shi, Wang, Cui, Yan, Xue: "Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury." in: Neural regeneration research, Vol. 7, Issue 10, pp. 741-8, (2015) (PubMed).

Luo, Liu, Chen, Wang, Zeng, Chen, Cheng et al.: "Nerve growth factor and inducible nitric oxide synthase expression in the mesencephalon and diencephalon, as well as visual- and auditory-related nervous tissues, in a macaque model of type 2 ..." in: Neural regeneration research, Vol. 7, Issue 4, pp. 302-7, (2015) (PubMed).

Huang, Zhang, Xu, Li, Zhao: "Expression of nerve growth factor in the prostate of male rats in response to chronic stress and sympathetic denervation." in: Experimental and therapeutic medicine, Vol. 8, Issue 4, pp. 1237-1240, (2014) (PubMed).

Pu, Shi, Wang, Wang, Guo, Liu, Sun, Gao, Zhou: "Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats." in: Journal of biomedical research, Vol. 25, Issue 4, pp. 292-8, (2013) (PubMed).

Orita, Koshi, Mitsuka, Miyagi, Inoue, Arai, Ishikawa, Hanaoka, Yamashita, Yamashita, Eguchi, Toyone, Takahashi, Ohtori: "Associations between proinflammatory cytokines in the synovial fluid and radiographic grading and pain-related scores in 47 consecutive patients with osteoarthritis of the knee." in: BMC musculoskeletal disorders, Vol. 12, pp. 144, (2012) (PubMed).

Details zu Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Andere Bezeichnung: NGF

Hintergrund:

Synonyms: Beta-nerve growth factor,Beta-NGF,NGF,NGFB,

Tissue Specificity: Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum.

Background: Nerve growth factor is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. The nucleotide sequence of human and mouse beta-NGF are very similar. The beta-subunits of nerve growth factor (NGFB) have been assigned to mouse chromosome 3 and human chromosome 1p22. The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1. A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception.

Molekulargewicht: 13 kDa

Gen-ID: 4803

UniProt: P01138

Pathways: Regulation of Cell Size