HPSE Antikörper (AA 206-457)

Produktnummer ABIN5518844

Dieses Anti-HPSE-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von HPSE in WB. Geeignet für Maus. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für HPSE Antikörper (AA 206-457) (ABIN5518844)

Target: HPSE (Heparanase (HPSE))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HPSE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-HPSE Antikörper

Bindungsspezifität: AA 206-457

Verwendungszweck: Anti-Heparanase 1/Hpse Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Heparanase 1/Hpse Antibody Picoband® (ABIN5518844). Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Heparanase 1 recombinant protein (Position: K206-K457). Mouse Heparanase 1 shares 80.2% and 96.4% amino acid (aa) sequence identity with human and rat Heparanase 1, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse
1. Hulett MD, Freeman C, Hamdorf BJ, Baker RT, Harris MJ, Parish CR (July 1999), "Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis", Nature medicine 5 (7): 803-9. 2. Nakajima M, Irimura T, Nicolson GL. (1988), "Heparanases and tumor metastasis", J. Cell. Biochem. 36 (2): 157-167. 3. Toyoshima, M., Nakajima, M. : Human heparanase: purification, characterization, cloning, and expression. J. Biol. Chem. 274: 24153-24160, 1999. 4. Vlodavsky I, Friedmann Y, Elkin M, Aingorn H, Atzmon R, Ishai-Michaeli R, Bitan M, Pappo O, Peretz T, Michal I, Spector L, Pecker I (July 1999), "Mammalian heparanase: gene cloning, expression and function in tumor progression and metastasis", Nature medicine 5 (7): 793-802. 5. Vlodavsky I, Goldshmidt O, Zcharia E, et al. (2003), "Mammalian heparanase: involvement in cancer metastasis, angiogenesis and normal development.", Semin. Cancer Biol. 12 (2): 121-9.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-HPSE Antikörper (ABIN5518844)

Qin, Xu, Qi, Guo, Wang, Xu, Qiao, Gu, Niu: "Evaluation of the C-domain of heparanase during AGE-induced macrophage inflammatory response." in: Experimental and therapeutic medicine, Vol. 14, Issue 2, pp. 1017-1022, (2017) (PubMed).

Huining, Yi, Dihong, Yifeng, Man, Ting, Jingting: "Inhibition of choriocarcinoma by Fe3O4-dextran-anti-β-human chorionic gonadotropin nanoparticles containing antisense oligodeoxynucleotide of heparanase." in: International journal of nanomedicine, Vol. 8, pp. 4371-8, (2014) (PubMed).

Details zu HPSE

Target: HPSE (Heparanase (HPSE))

Andere Bezeichnung: Hpse

Hintergrund:

Synonyms: Heparanase,3.2.1.166,Endo-glucoronidase,Heparanase 8 kDa subunit,Heparanase 50 kDa subunit,Hpse,Hpa,

Tissue Specificity: Expressed in skin, mainly in the stratum granulosum and the first layer of the stratum corneum in the upper part of the epidermis. Also detected in hair follicles and in sebaceous glands. .

Background: Heparanase, also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides. Heparanase is an endo-beta-D-glucuronidase capable of cleaving heparan sulfate and has been implicated in inflammation and tumor angiogenesis and metastasis. The successful penetration of the endothelial cell layer that lines the interior surface of blood vessels is an important process in the formation of blood borne tumour metastases. Heparan sulfate proteoglycans are major constituents of this layer and it has been shown that increased metastatic potential corresponds with increased heparanase activity for a number of cell lines.

Molekulargewicht: 65 kDa

Gen-ID: 15442

Pathways: Glycosaminoglycan Metabolic Process