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PPL Antikörper (C-Term)

Dieses Anti-PPL-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PPL in WB, IHC, IF, ICC und FACS. Geeignet für Human, Maus und Ratte.
Produktnummer ABIN5518787

Kurzübersicht für PPL Antikörper (C-Term) (ABIN5518787)

Target

Alle PPL Antikörper anzeigen
PPL (Periplakin (PPL))

Reaktivität

  • 33
  • 9
  • 5
  • 1
Human, Maus, Ratte

Wirt

  • 35
  • 2
Kaninchen

Klonalität

  • 37
Polyklonal

Konjugat

  • 28
  • 3
  • 2
  • 2
  • 1
  • 1
Dieser PPL Antikörper ist unkonjugiert

Applikation

  • 35
  • 24
  • 11
  • 10
  • 9
  • 5
  • 4
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 8
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1664-1701, C-Term

    Verwendungszweck

    Anti-Periplakin/PPL Antibody Picoband®

    Sequenz

    DTGRELSPEE AHRAGLIDWN MFVKLRSQEC DWEEISVK

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-Periplakin/PPL Antibody Picoband® (ABIN5518787). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminus of human Periplakin, different from the related mouse sequence by one amino acid.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. "Entrez Gene: PPL periplakin". 2. Aho S, McLean WH, Li K, Uitto J (Jun 1998). "cDNA cloning, mRNA expression, and chromosomal mapping of human and mouse periplakin genes". Genomics. 48 (2): 242-7. 3. Kazerounian, Shideh, Uitto Jouni, Aho Sirpa (Oct 2002). "Unique role for the periplakin tail in intermediate filament association: specific binding to keratin 8 and vimentin". Exp. Dermatol. Denmark. 11(5): 428-38.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    PPL (Periplakin (PPL))

    Andere Bezeichnung

    PPL

    Hintergrund

    Synonyms: Periplakin,190 kDa paraneoplastic pemphigus antigen,195 kDa cornified envelope precursor protein,PPL,KIAA0568,

    Tissue Specificity: Expressed in stratified squamous epithelia and in some other epithelia. .

    Background: Periplakin is a protein that in humans is encoded by the PPL gene. The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.

    Molekulargewicht

    202 kDa

    Gen-ID

    5493

    UniProt

    O60437
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