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PPL Antikörper (C-Term)

PPL Reaktivität: Human, Maus, Ratte WB, IHC, IF, ICC, FACS Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN5518787
  • Target Alle PPL Antikörper anzeigen
    PPL (Periplakin (PPL))
    Bindungsspezifität
    • 8
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1664-1701, C-Term
    Reaktivität
    • 33
    • 9
    • 5
    • 1
    Human, Maus, Ratte
    Wirt
    • 35
    • 2
    Kaninchen
    Klonalität
    • 37
    Polyklonal
    Konjugat
    • 28
    • 3
    • 2
    • 2
    • 1
    • 1
    Dieser PPL Antikörper ist unkonjugiert
    Applikation
    • 35
    • 24
    • 11
    • 10
    • 9
    • 5
    • 4
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Verwendungszweck
    Anti-Periplakin/PPL Antibody Picoband®
    Sequenz
    DTGRELSPEE AHRAGLIDWN MFVKLRSQEC DWEEISVK
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins
    Produktmerkmale
    Anti-Periplakin/PPL Antibody Picoband® (ABIN5518787). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the C-terminus of human Periplakin, different from the related mouse sequence by one amino acid.
    Isotyp
    IgG
  • Applikationshinweise
    Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. "Entrez Gene: PPL periplakin". 2. Aho S, McLean WH, Li K, Uitto J (Jun 1998). "cDNA cloning, mRNA expression, and chromosomal mapping of human and mouse periplakin genes". Genomics. 48 (2): 242-7. 3. Kazerounian, Shideh, Uitto Jouni, Aho Sirpa (Oct 2002). "Unique role for the periplakin tail in intermediate filament association: specific binding to keratin 8 and vimentin". Exp. Dermatol. Denmark. 11(5): 428-38.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target
    PPL (Periplakin (PPL))
    Andere Bezeichnung
    PPL (PPL Produkte)
    Hintergrund

    Synonyms: Periplakin,190 kDa paraneoplastic pemphigus antigen,195 kDa cornified envelope precursor protein,PPL,KIAA0568,

    Tissue Specificity: Expressed in stratified squamous epithelia and in some other epithelia. .

    Background: Periplakin is a protein that in humans is encoded by the PPL gene. The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.

    Molekulargewicht
    202 kDa
    Gen-ID
    5493
    UniProt
    O60437
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