GAPDH Antikörper (AA 136-335)

Produktnummer ABIN5518757

Dieses Anti-GAPDH-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GAPDH in WB, IHC, IF, ICC und FACS. Geeignet für Human, Maus, Ratte, Huhn, Affe und Zebrafisch (Danio rerio). Dieses Primary Antibody wurde in 115+ Publikationen zitiert.

Kurzübersicht für GAPDH Antikörper (AA 136-335) (ABIN5518757)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Human, Maus, Ratte, Huhn, Affe, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAPDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-GAPDH Antikörper

Bindungsspezifität: AA 136-335

Verwendungszweck: Anti-GAPDH Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-GAPDH Antibody Picoband® (ABIN5518757). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human GAPDH recombinant protein (Position: N136-E335). Human GAPDH shares 95% and 94.5% amino acid (aa) sequence identity with mouse and rat GAPDH, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat, Chicken, Zebrafish
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Bae, B.-I., Hara, M. R., Cascio, M. B., Wellington, C. L., Hayden, M. R., Ross, C. A., Ha, H. C., Li, X.-J., Snyder, S. H., Sawa, A. Mutant Huntingtin: nuclear translocation and cytotoxicity mediated by GAPDH. Proc. Nat. Acad. Sci. 103: 3405-3409, 2006. 2. Colell, A., Ricci, J.-E., Tait, S., Milasta, S., Maurer, U., Bouchier-Hayes, L., Fitzgerald, P., Guio-Carrion, A., Waterhouse, N. J., Li, C. W., Mari, B., Barbry, P., Newmeyer, D. D., Beere, H. M., Green, D. R. GAPDH and autophagy preserve survival after apoptotic cytochrome c release in the absence of caspase activation. Cell 129: 983-997, 2007. Note: Erratum: Cell 130: 385 only, 2007. 3. Tarze A, Deniaud A, Le Bras M, Maillier E, Molle D, Larochette N, Zamzami N, Jan G, Kroemer G, Brenner C (April 2007). "GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization".Oncogene 26 (18): 2606-20.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-GAPDH Antikörper (ABIN5518757)

Wang, Xue, Zhang, Bu, Zhu, Lai: "Autophagy protects ovarian cancer-associated fibroblasts against oxidative stress." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 10, pp. 1376-85, (2018) (PubMed).

Duan, Man, Tang, Yao, Tao, Yu, Liang, Makawana, Zou, Wang, Zhu: "Design, Synthesis and Antitumor Activity of Novel link-bridge and B-Ring Modified Combretastatin A-4 (CA-4) Analogues as Potent Antitubulin Agents." in: Scientific reports, Vol. 6, pp. 25387, (2018) (PubMed).

Liu, Kuang, Wu, Jin, Sun: "A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo." in: Scientific reports, Vol. 6, pp. 26722, (2018) (PubMed).

Zou, Duan, Wang, Gao, Chen, Ou, Liang, Wang, Yuan, Wang, Zhu: "DYT-40, a novel synthetic 2-styryl-5-nitroimidazole derivative, blocks malignant glioblastoma growth and invasion by inhibiting AEG-1 and NF-κB signaling pathways." in: Scientific reports, Vol. 6, pp. 27331, (2018) (PubMed).

Guo, Li, Qian, Bi, He, Jin, Luo, Li, Meng, Li: "TGEV infection up-regulates FcRn expression via activation of NF-κB signaling." in: Scientific reports, Vol. 6, pp. 32154, (2018) (PubMed).

Li, Chen, Xiao, Li, Wang, Su, Guo, Liu, Yan, Yang, Zhang, Bu: "Mouse Sirt3 promotes autophagy in AngII-induced myocardial hypertrophy through the deacetylation of FoxO1." in: Oncotarget, Vol. 7, Issue 52, pp. 86648-86659, (2018) (PubMed).

Feng, Zhang, Zhou, Zhou, Li, Guo, Feng, Ma, Huang, Huang: "mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27." in: Oncotarget, Vol. 8, Issue 4, pp. 6304-6318, (2018) (PubMed).

Li, Guan, Liu, Zhao, Li, Zhang, Tan, Mi, Li: "Epoxyeicosanoids prevent intervertebral disc degeneration in vitro and in vivo." in: Oncotarget, Vol. 8, Issue 3, pp. 3781-3797, (2018) (PubMed).

Hu, Cao, Pang, Luo, Zou, Feng, Li, Chen, Huang: "Tumor grade related expression of neuroglobin is negatively regulated by PPARγ and confers antioxidant activity in glioma progression." in: Redox biology, Vol. 12, pp. 682-689, (2018) (PubMed).

Liao, Xing, Xu, Liu, Zhang: "Evaluation of the circulating level of fibroblast activation protein α for diagnosis of esophageal squamous cell carcinoma." in: Oncotarget, Vol. 8, Issue 18, pp. 30050-30062, (2018) (PubMed).

Cai, Tan, Li, Gao, Li, Li, Tang, Li, Hui: "Glucagon-Like Peptide-1 (GLP-1) Treatment Ameliorates Cognitive Impairment by Attenuating Arc Expression in Type 2 Diabetic Rats." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 4334-4342, (2018) (PubMed).

Wu, Sun, Ye, Zhang, Guo, Huang, Jing, Xiang, Yu, Guo: "Iguratimod prevents ovariectomy‑induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator‑activated receptor‑γ." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 8200-8208, (2018) (PubMed).

Liu, Liu, Tan, Tang, Long, Li, Liang, Chu, Yang: "Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 8953-8963, (2018) (PubMed).

Wu, Xing, Zhang, Mao, Chen, Liang, Wang, Ren, Cui, Jiang, Wang, Zou, Ji: "Autophagy promotes fibrosis and apoptosis in the peritoneum during long-term peritoneal dialysis." in: Journal of cellular and molecular medicine, Vol. 22, Issue 2, pp. 1190-1201, (2018) (PubMed).

Gu, Fang, Kang, Hu, Yu, Li, Cheng, Gao: "Effect of ALDH2 on High Glucose-Induced Cardiac Fibroblast Oxidative Stress, Apoptosis, and Fibrosis." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 9257967, (2018) (PubMed).

Li, Wang, Qin, Fan, Tian, Liu: "Fingolimod suppresses neuronal autophagy through the mTOR/p70S6K pathway and alleviates ischemic brain damage in mice." in: PLoS ONE, Vol. 12, Issue 11, pp. e0188748, (2018) (PubMed).

Zou, Zhang, Yuan, Yang, Wu, Song, Zhu, Mao, Chen: "Endogenous hormone 2-methoxyestradiol suppresses venous hypertension-induced angiogenesis through up- and down-regulating p53 and id-1." in: Journal of cellular and molecular medicine, Vol. 22, Issue 2, pp. 957-967, (2018) (PubMed).

Huang, Pan, Mao, Zhang, Ma, Xi, You: "Sinapic Acid Inhibits the IL-1β-Induced Inflammation via MAPK Downregulation in Rat Chondrocytes." in: Inflammation, Vol. 41, Issue 2, pp. 562-568, (2018) (PubMed).

Miao, Margolis, Gao, Voss, Li, Martini, Norton, Bossé, Wankowicz, Cullen, Horak, Wind-Rotolo, Tracy, Giannakis, Hodi, Drake, Ball, Allaf, Snyder, Hellmann, Ho, Motzer, Signoretti, Kaelin, Choueiri et al.: "Genomic correlates of response to immune checkpoint therapies in clear cell renal cell carcinoma. ..." in: Science (New York, N.Y.), Vol. 359, Issue 6377, pp. 801-806, (2018) (PubMed).

Yang, Zhang, Li, Wen: "MALAT1 enhanced the proliferation of human osteoblasts treated with ultra‑high molecular weight polyethylene by targeting VEGF via miR‑22‑5p." in: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1536-1546, (2018) (PubMed).

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: GAPDH

Hintergrund:

Synonyms: Glyceraldehyde-3-phosphate dehydrogenase,GAPDH,1.2.1.12,Peptidyl-cysteine S-nitrosylase GAPDH,2.6.99.-,GAPDH,GAPD,CDABP0047, OK/SW-cl.12,

Tissue Specificity: Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.

Background: Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37 kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH is mapped to 12p13.31. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus.

Molekulargewicht: 36 kDa

Gen-ID: 2597

UniProt: P04406