APEX2 Antikörper (AA 102-210)

Produktnummer ABIN5518737

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch APEX2 in WB und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Affe.

Kurzübersicht für APEX2 Antikörper (AA 102-210) (ABIN5518737)

Target: APEX2 (APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APEX2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-APEX2 Antikörper

Bindungsspezifität: AA 102-210

Verwendungszweck: Anti-APEX2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-APEX2 Antibody Picoband® (ABIN5518737). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human APEX2 recombinant protein (Position: L102-A210). Human APEX2 shares 91.7% amino acid (aa) sequence identity with mouse APEX2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Monkey, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Burkovics, P., Szukacsov, V., Unk, I., Haracska, L.Human Ape2 protein has 3-5-prime exonuclease activity that acts preferentially on mismatched base pairs.Nucleic Acids Res. 34: 2508-2515, 2006. 2. Hadi, M. Z., Ginalski, K., Nguyen, L. H., Wilson, D. M., III.Determinants in nuclease specificity of Ape1 and Ape2, human homologues of Escherichia coli exonuclease III.J. Molec. Biol. 316: 853-866, 2002. 3. Tsuchimoto, D., Sakai, Y., Sakumi, K., Nishioka, K., Sasaki, M., Fujiwara, T., Nakabeppu, Y.Human APE2 protein is mostly localized in the nuclei and to some extent in the mitochondria, while nuclear APE2 is partly associated with proliferating cell nuclear antigen.Nucleic Acids Res. 29: 2349-2360, 2001.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu APEX2

Target: APEX2 (APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2))

Andere Bezeichnung: APEX2

Hintergrund:

Synonyms: DNA- (apurinic or apyrimidinic site) lyase 2,3.1.-.-,4.2.99.18,AP endonuclease XTH2,APEX nuclease 2,APEX nuclease-like 2,Apurinic-apyrimidinic endonuclease 2,AP endonuclease 2,APEX2,APE2, APEXL2, XTH2,

Tissue Specificity: Highly expressed in brain and kidney. Weakly expressed in the fetal brain. .

Background: APEX2, also called apurinic/apyrimidinic endonuclease like-2, is a member of the apurinic/apyrimidinic (AP) family of endonucleases that initiate the repair of AP sites formed by spontaneous hydrolysis of the N-glycosylic bond, mutagen-induced base release, or damaged-base excision by a DNA repair glycosylase. RT-PCR detected APEX2 expression in HeLa cells, Jurkat cells, and human kidney, brain and fetal brain tissue. The APEX2 gene is mapped to chromosome Xp11.21. APEX2 participates in both nuclear and mitochondrial base excision repair (BER) and it can play a role in processing 3-prime-damaged termini or 3-prime-mismatched nucleotides. Additionally, APEX2 displayed weaker AP site-specific and 3-prime nuclease activities compared to APEX1.

Molekulargewicht: 57 kDa

Gen-ID: 27301