COL1A1 Antikörper (C-Term)

Produktnummer ABIN5518708

Produktdetails anti-COL1A1 Antikörper

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Bindungsspezifität: AA 1192-1207, C-Term

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL1A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for Collagen alpha-1(I) chain(COL1A1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequenz: QPPQEKSQDG GRYYRA

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Collagen alpha-1(I) chain(COL1A1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: collagen, type I, alpha 1
Protein Name: Collagen alpha-1(I) chain

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I(1192-1207aa QPPQEKSQDGGRYYRA), identical to the related rat sequence, and different from the related human sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for COL1A1 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Predicted Species: Human, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-COL1A1 Antikörper (ABIN5518708)

Tang, Pei, Yang, Wang, Wang, Gao, Li, Yang, Yang: "The inhibition of calpains ameliorates vascular restenosis through MMP2/TGF-β1 pathway." in: Scientific reports, Vol. 6, pp. 29975, (2018) (PubMed).

Yao, Sun, Fei, Wang, Wang, Zhang, Wu, Liu, Liu, Cui, Li, Yu, Dang, Wang: "Screening in larval zebrafish reveals tissue-specific distribution of fifteen fluorescent compounds." in: Disease models & mechanisms, Vol. 10, Issue 9, pp. 1155-1164, (2018) (PubMed).

Shang, Liu, Liu, Li, Dou, Zhang, Sun, Zhang, Zhu, Mu, Ding, Wen: "Telmisartan improves vascular remodeling through ameliorating prooxidant and profibrotic mechanisms in hypertension via the involvement of transforming growth factor-β1." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 4537-4544, (2018) (PubMed).

Zhou, Zhang, Gao, Tan, Zheng, Zhao, Zhang: "Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-β1/Smad pathway." in: International journal of molecular medicine, Vol. 41, Issue 1, pp. 473-484, (2018) (PubMed).

Yang, Jia, Liu, Ma: "Effect of genistein on myocardial fibrosis in diabetic rats and its mechanism." in: Molecular medicine reports, Vol. 17, Issue 2, pp. 2929-2936, (2018) (PubMed).

"Retraction: Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction." in: PLoS ONE, Vol. 13, Issue 2, pp. e0192465, (2018) (PubMed).

Jiang, Zhong, Zheng, Zhao: "Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro." in: International journal of molecular medicine, Vol. 41, Issue 4, pp. 2150-2158, (2018) (PubMed).

Zhang, Ju, Nie, Song, Xu, Gao: "Adenovirus‑mediated knockdown of activin A receptor type 2A attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced activation of primary hepatic stellate cells." in: International journal of molecular medicine, Vol. 42, Issue 1, pp. 279-289, (2018) (PubMed).

Zhao, Wang, Wang, Hong, Miao, Liao, Zhou, Liu: "An essential role for Wnt/β-catenin signaling in mediating hypertensive heart disease." in: Scientific reports, Vol. 8, Issue 1, pp. 8996, (2018) (PubMed).

Chen, Kong, Liu, Gao, Zhang, Li, Liu: "In vitro differentiation of endometrial regenerative cells into smooth muscle cells: Α potential approach for the management of pelvic organ prolapse." in: International journal of molecular medicine, Vol. 38, Issue 1, pp. 95-104, (2017) (PubMed).

Zhao, Peng, Cui, Guo, Yan: "Dimethyl α-ketoglutarate reduces CCl4-induced liver fibrosis through inhibition of autophagy in hepatic stellate cells." in: Biochemical and biophysical research communications, Vol. 481, Issue 1-2, pp. 90-96, (2017) (PubMed).

Huang, Jin, Lin, Lin, Zheng, Zhao: "Beneficial effects of sulfonamide‑based gallates on osteoblasts in vitro." in: Molecular medicine reports, Vol. 15, Issue 3, pp. 1149-1156, (2017) (PubMed).

et al.: "Erratum: Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway ..." in: International journal of nanomedicine, Vol. 12, pp. 1515, (2017) (PubMed).

Cao, Shi, Ge: "miR-21 enhances cardiac fibrotic remodeling and fibroblast proliferation via CADM1/STAT3 pathway." in: BMC cardiovascular disorders, Vol. 17, Issue 1, pp. 88, (2017) (PubMed).

Huang, Wu, Wang, Zheng, Zhao: "Protective effects of baicalin on rabbit articular chondrocytes in vitro." in: Experimental and therapeutic medicine, Vol. 13, Issue 4, pp. 1267-1274, (2017) (PubMed).

Cheng, Ge, Lan, Ma, Chi, Kuang, Sun, Zhao, Liu, Feng, Huang, Luo, Li, Zhang, Hu, Xu, Liu, Huo, Deng, Yang, Xi, Zhang, Siegenthaler, Chen: "Zoledronate dysregulates fatty acid metabolism in renal tubular epithelial cells to induce nephrotoxicity." in: Archives of toxicology, Vol. 92, Issue 1, pp. 469-485, (2017) (PubMed).

Wang, Shi, Xu, Qin, Wang: "Hesperetin alleviates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro." in: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 3713-3719, (2017) (PubMed).

Cui, Tang, Li, Wang, Rao, Wang, Liu, Chen: "Lipoxin A4 improves erectile dysfunction in rats with type I diabetes by inhibiting oxidative stress and corporal fibrosis." in: Asian journal of andrology, Vol. 20, Issue 2, pp. 166-172, (2017) (PubMed).

Wang, Yao, Shen, Ding, Shi, Chen, He, Ding, Zhang, Xie: "FOXA2 alleviates CCl4-induced liver fibrosis by protecting hepatocytes in mice." in: Scientific reports, Vol. 7, Issue 1, pp. 15532, (2017) (PubMed).

Zhang, Jin, Tian, Wu: "hsa-miR-29c-3p regulates biological function of colorectal cancer by targeting SPARC." in: Oncotarget, Vol. 8, Issue 61, pp. 104508-104524, (2017) (PubMed).

Details zu COL1A1

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Andere Bezeichnung: COL1A1 (COL1A1 Produkte)

Hintergrund: Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Synonyms: Alpha 1 type I collagen antibody|Alpha 2 type I collagen antibody|Alpha-1 type I collagen antibody|CO1A1_HUMAN antibody|COL1A1 antibody|COL1A2 antibody|Collagen alpha-1(I) chain antibody|Collagen I alpha 1 polypeptide antibody|Collagen I alpha 2 polypeptide antibody|Collagen Of Skin Tendon And Bone antibody|Collagen Type 1 antibody|Collagen type I alpha 1 antibody|Collagen type I alpha 2 antibody|OI4 antibody|Osteogenesis Imperfecta Type IV antibody|Pro alpha 1(I) collagen antibody|Type I procollagen antibody

UniProt: P11087

Pathways: Sensory Perception of Sound, Autophagie, Growth Factor Binding