Hexosaminidase A Antikörper (C-Term)

Produktnummer ABIN5518688

Produktdetails anti-Hexosaminidase A Antikörper

Target: Hexosaminidase A (HEXA)

Bindungsspezifität: AA 513-529, C-Term

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Hexosaminidase A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Rabbit IgG polyclonal antibody for Beta-hexosaminidase subunit alpha(HEXA) detection. Tested with WB in Human.

Sequenz: QAQPLNVGFC EQEFEQT

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Beta-hexosaminidase subunit alpha(HEXA) detection. Tested with WB in Human.
Gene Name: hexosaminidase A(alpha polypeptide)
Protein Name: Beta-hexosaminidase subunit alpha

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HEXA(513-529aa QAQPLNVGFCEQEFEQT), different from the related mouse sequence by three amino acids, and from the related rat sequences by four amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu Hexosaminidase A

Target: Hexosaminidase A (HEXA)

Andere Bezeichnung: HEXA (HEXA Produkte)

Synonyme: TSD antikoerper, fc04h11 antikoerper, wu:fc04h11 antikoerper, wu:fv09f06 antikoerper, si:dkey-35i22.2 antikoerper, HEXA antikoerper, Hex-1 antikoerper, zgc:112084 antikoerper, hexosaminidase subunit alpha antikoerper, hexosaminidase B (beta polypeptide) antikoerper, CUGBP Elav-like family member 6 antikoerper, hexosaminidase A antikoerper, hexosaminidase A (alpha polypeptide) antikoerper, beta-hexosaminidase subunit alpha-like antikoerper, HEXA antikoerper, hexb antikoerper, CELF6 antikoerper, Hexa antikoerper, hexa antikoerper, LOC100228061 antikoerper

Hintergrund: HEXA(hexosaminidase A(alpha polypeptide)) is an enzyme that in humans is encoded by the HEXA gene. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines The HEXA gene encodes the alpha subunit of hexosaminidase A, a lysosomal enzyme involved in the breakdown of gangliosides. The HEXA gene is mapped on 15q23. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit is able to hydrolyze GM2 gangliosides. The alpha subunit contains a key residue, Arg-424, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides. Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein. Their results allowed them to define 2 noncontiguous sequences in the alpha subunit(amino acids 1-191 and 403-529) which, when substituted into analogous positions in the beta subunit, conferred activity against the sulfated substrate.

Synonyms: Beta hexosaminidase alpha chain precursor antibody|Beta hexosaminidase subunit alpha antibody|Beta N acetylhexosaminidase antibody|Beta N acetylhexosaminidase subunit alpha antibody|Beta-hexosaminidase subunit alpha antibody|Beta-N-acetylhexosaminidase subunit alpha antibody|Hexa antibody|HEXA_HUMAN antibody|Hexosaminidase A(alpha polypeptide) antibody|Hexosaminidase A alpha polypeptide antibody|Hexosaminidase A antibody|Hexosaminidase subunit A antibody|MGC99608 antibody|N acetyl beta glucosaminidase antibody|N acetyl beta glucosaminidase subunit alpha antibody|N-acetyl-beta-glucosaminidase subunit alpha antibody|TSD antibody

UniProt: P06865

Pathways: Sensory Perception of Sound, Glycosaminoglycan Metabolic Process