HSD11B1 Antikörper (N-Term)

Produktnummer ABIN5518666

Der Kaninchen Polyklonal Anti-HSD11B1-Antikörper wurde für WB und IHC validiert. Er ist geeignet, HSD11B1 in Proben von Human, Ratte, Maus und Affe zu detektieren.

Kurzübersicht für HSD11B1 Antikörper (N-Term) (ABIN5518666)

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Reaktivität: Human, Ratte, Maus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSD11B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-HSD11B1 Antikörper

Bindungsspezifität: AA 16-33, N-Term

Verwendungszweck: Anti-HSD11B1 Antibody Picoband®

Sequenz: MAYYYYSANE EFRPEMLQ

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-HSD11B1 Antibody (ABIN5518666). Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HSD11B1, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
1. "Entrez Gene: HSD11B1 hydroxysteroid (11-beta) dehydrogenase 1".

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu HSD11B1

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Andere Bezeichnung: HSD11B1

Hintergrund:

Synonyms: Corticosteroid 11-beta-dehydrogenase isozyme 1,1.1.1.146,11-beta-hydroxysteroid dehydrogenase 1,11-DH,11-beta-HSD1,Short chain dehydrogenase/reductase family 26C member 1,HSD11B1,HSD11, HSD11L, SDR26C1,

Tissue Specificity: Widely expressed. Highest expression in liver.

Background: 11beta-hydroxysteroid dehydrogenase type 1 is an NADPH-dependent enzyme highly expressed in key metabolic tissues including liver, adipose tissue, and the central nervous system. In these tissues, HSD11B1 reduces cortisone to the active hormone cortisol that activates glucocorticoid receptors. It is inhibited by carbenoxolone, a drug typically used in the treatment of peptic ulcers. Tannin et al. (1991) localized the HSD11B1 gene to chromosome 1. The localization was confirmed by isolating the gene from a chromosome 1-specific library using the cDNA as a probe. Schutte et al. (2000) mapped the HSD11B1 gene to 1q32-q41.

Sequence Similarities: Belongs to the connexin family. Beta-type (group I) subfamily.

Molekulargewicht: 36 kDa

UniProt: P28845

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Carbohydrate Metabolic Process