ALPL Antikörper (N-Term)

Produktnummer ABIN5518639

Der Kaninchen Polyklonal Anti-ALPL-Antikörper wurde für WB validiert. Er ist geeignet, ALPL in Proben von Maus und Ratte zu detektieren. Es sind 20+ Publikationen verfügbar.

Kurzübersicht für ALPL Antikörper (N-Term) (ABIN5518639)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ALPL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-ALPL Antikörper

Bindungsspezifität: AA 18-32, N-Term

Verwendungszweck: Anti-Alkaline Phosphatase/ALPL Antibody Picoband®

Sequenz: FVPEKEKDPS YWRQQ

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Alkaline Phosphatase/ALPL Antibody (ABIN5518639). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of rat Alkaline Phosphatase, different from the related mouse sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Rat, Mouse
1. Weiss MJ, Henthorn PS, Lafferty MA, Slaughter C, Raducha M, Harris H (Oct 1986). "Isolation and characterization of a cDNA encoding a human liver/bone/kidney-type alkaline phosphatase". Proc Natl Acad Sci U S A 83 (19): 7182-6. 2. Swallow DM, Povey S, Parkar M, Andrews PW, Harris H, Pym B, Goodfellow P (May 1988). "Mapping of the gene coding for the human liver/bone/kidney isozyme of alkaline phosphatase to chromosome 1". Ann Hum Genet 50 (Pt 3): 229-35. 3. "Entrez Gene: ALPL alkaline phosphatase, liver/bone/kidney" 4. Fedde, K. N., Whyte, M. P. Alkaline phosphatase (tissue-nonspecific isoenzyme) is a phosphoethanolamine and pyridoxal-5-prime-phosphate ectophosphatase: normal and hypophosphatasia fibroblast study. Am. J. Hum. Genet. 47: 767-775, 1990.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-ALPL Antikörper (ABIN5518639)

Sun, Jiang, Chen, Xue, Mao, Ruan, Song, Mustea: "Decreasing the ratio of matriptase/HAI‑1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer." in: Molecular medicine reports, Vol. 14, Issue 2, pp. 1465-74, (2017) (PubMed).

Hou, Huang, Luo, Wang, Liu, Deng, Zhang, Liu, Chen: "MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR." in: American journal of translational research, Vol. 9, Issue 11, pp. 4963-4973, (2017) (PubMed).

Liu, Deng, Zhang, Zhang: "Wnt3a expression during the differentiation of adipose-derived stem cells into cholinergic neurons." in: Neural regeneration research, Vol. 7, Issue 19, pp. 1463-8, (2015) (PubMed).

Xu, Dong, Huang, Li, Qin, Ren, Guo, Chen, Huang: "Decreased osteoclastogenesis, osteoblastogenesis and low bone mass in a mouse model of type 2 diabetes." in: Molecular medicine reports, Vol. 10, Issue 4, pp. 1935-41, (2014) (PubMed).

Liu, Lu, Wu, Yan: "A multi-biomarker assessment of single and combined effects of norfloxacin and sulfamethoxazole on male goldfish (Carassius auratus)." in: Ecotoxicology and environmental safety, Vol. 102, pp. 12-7, (2014) (PubMed).

Guo, Shi, Liu, Lin, Bi, Shi, Yan: "Cartilage oligomeric matrix protein gene multilayers inhibit osteogenic differentiation and promote chondrogenic differentiation of mesenchymal stem cells." in: International journal of molecular sciences, Vol. 15, Issue 11, pp. 20117-33, (2014) (PubMed).

Feng, Zhu, Zhang, Jia, Cheng, Ding, Zhu et al.: "Amelioration of compound 4,4'-diphenylmethane-bis(methyl)carbamate on high mobility group box1-mediated inflammation and oxidant stress responses in human umbilical vein endothelial cells via ..." in: International immunopharmacology, Vol. 15, Issue 2, pp. 206-16, (2013) (PubMed).

Shen, Zhan, Deng, Ren, Ye, Xiao, Guo: "MicroRNA-519a demonstrates significant tumour suppressive activity in laryngeal squamous cells by targeting anti-carcinoma HuR gene." in: The Journal of laryngology and otology, Vol. 127, Issue 12, pp. 1194-202, (2013) (PubMed).

Zhang, Wu, Hu, Wang, Li: "Rho kinase inhibitor Y-27632 and Accutase dramatically increase mouse embryonic stem cell derivation." in: In vitro cellular & developmental biology. Animal, Vol. 48, Issue 1, pp. 30-6, (2012) (PubMed).

Zhang, Yang, Jiang, Fang, Xiong, Cheng, Xia: "The lupus-derived anti-double-stranded DNA IgG contributes to myofibroblast-like phenotype in mesangial cells." in: Journal of clinical immunology, Vol. 32, Issue 6, pp. 1270-8, (2012) (PubMed).

Li, Xia, Tang, Wang, Bei, Zeng: "In vitro and in vivo biocompatibility investigation of diamond-like carbon coated nickel-titanium shape memory alloy." in: Artificial cells, blood substitutes, and immobilization biotechnology, Vol. 39, Issue 3, pp. 137-42, (2011) (PubMed).

Su, Wang, Qi, Zhao, Li, Cui: "Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts." in: Molecular vision, Vol. 17, pp. 247-56, (2011) (PubMed).

Zhao, Gao, Mei, Li, Wang: "Differentiation of human mesenchymal stem cells: the potential mechanism for estrogen-induced preferential osteoblast versus adipocyte differentiation." in: The American journal of the medical sciences, Vol. 341, Issue 6, pp. 460-8, (2011) (PubMed).

Wang, Huang, Feng, Chen, Tang, Chen: "Expression, purification, and characterization of functional recombinant human daintain/AIF-1 in Escherichia coli." in: Zeitschrift fu?r Naturforschung. C, Journal of biosciences, Vol. 65, Issue 11-12, pp. 726-32, (2011) (PubMed).

Lai, Yan, Wu, Leng, Ju: "Ultrasensitive multiplexed immunoassay with electrochemical stripping analysis of silver nanoparticles catalytically deposited by gold nanoparticles and enzymatic reaction." in: Analytical chemistry, Vol. 83, Issue 7, pp. 2726-32, (2011) (PubMed).

Shen, Ren, Ye, Guo, Kang, Ding: "Significance and relationship between DJ-1 gene and surviving gene expression in laryngeal carcinoma." in: European journal of histochemistry : EJH, Vol. 55, Issue 1, pp. e9, (2011) (PubMed).

Guan, Wang, Hou, Chen, Li, Yue, Ma: "Derivation and characteristics of pluripotent embryonic germ cells in duck." in: Poultry science, Vol. 89, Issue 2, pp. 312-7, (2010) (PubMed).

Song, Lu, Wang, Zhang, Xu, Qin: "Study on environmental estrogen pollution in Yangtze River (Nanjing section) by an in vivo bioassay." in: Bulletin of environmental contamination and toxicology, Vol. 84, Issue 4, pp. 406-12, (2010) (PubMed).

Lu, Song, Wang, Yan: "Assessment of in vivo estrogenic response and the identification of environmental estrogens in the Yangtze River (Nanjing section)." in: Chemosphere, Vol. 80, Issue 9, pp. 982-90, (2010) (PubMed).

Li, Yan, Han, Zhang: "Mimotope vaccination for epitope-specific induction of anti-CD20 antibodies." in: Cellular immunology, Vol. 239, Issue 2, pp. 136-43, (2006) (PubMed).

Details zu ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Andere Bezeichnung: ALPL

Hintergrund:

Synonyms: Alkaline phosphatase, tissue-nonspecific isozyme,AP-TNAP,TNSALP,3.1.3.1,Alkaline phosphatase liver/bone/kidney isozyme,Alpl,

Tissue Specificity: Widely expressed, with highest expression in brain. .

Background: Alkaline phosphatase, tissue-nonspecific isozyme is an enzyme that in humans is encoded by the ALPL gene. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. Liver/bone/kidney alkaline phosphatase, also known as tissue-nonspecific alkaline phosphatase, acts physiologically as a lipid-anchored phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP) ectophosphatase Mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked ly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects.

Sequence Similarities: Contains 12 fibronectin type-I domains.

Molekulargewicht: 57 kDa

UniProt: P08289