SMT1 Antikörper

Produktnummer ABIN5326722

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SMT1 in IL und WB. Er zeigt eine Reaktivität gegenüber Arabidopsis thaliana und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für SMT1 Antikörper (ABIN5326722)

Target: SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))

Reaktivität: Arabidopsis thaliana

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunolocalization (IL), Western Blotting (WB)

Produktdetails anti-SMT1 Antikörper

Kreuzreaktivität (Details): Not reactive in: Hordeum vulgare, Triticum aestivum, Withania somnifera

Homologie: Amborella trichopoda, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium mexicanum, Medicago truncatula, Populus trichopocarpa, Prunus persica, Ricinus communis, Theobroma cacao, Vitis vinifera,

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 38 kDa

Aufreinigung: affinity purified

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 Q9LM02, At5g13710

Anwendungsinformationen

Applikationshinweise: 1: 500 - 1:1 000 with ECL Advance or + (WB), 1: 50 - 1: 100 (IL)

Kommentare:

SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutté Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 μL of sterile water.

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Referenzen für anti-SMT1 Antikörper (ABIN5326722)

Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).

Boutté, Frescatada-Rosa, Men, Chow, Ebine, Gustavsson, Johansson, Ueda, Moore, Jürgens, Grebe: "Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis." in: The EMBO journal, Vol. 29, Issue 3, pp. 546-58, (2010) (PubMed).

Details zu SMT1

Target: SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))

Andere Bezeichnung: Sterol methyltransferase 1 (SMT1)

Hintergrund: AGI Code: At5g13710
SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD

Molekulargewicht: 38 kDa

UniProt: Q9LM02