SMT1 Antikörper

Produktnummer ABIN5326722

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch SMT1 in IL und WB. Es zeigt Reaktivität gegenüber Proben von Arabidopsis thaliana. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für SMT1 Antikörper (ABIN5326722)

Target: SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))

Reaktivität: Arabidopsis thaliana

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunolocalization (IL), Western Blotting (WB)

Produktdetails anti-SMT1 Antikörper

Verwendungszweck: Anti-SMT1 | Sterol methyltransferase 1

Kreuzreaktivität (Details): Arabidopsis thaliana
Not reactive in: Hordeum vulgare, Triticum aestivum, Solanum lycopersicum, Withania somnifera

Homologie: Amborella trichopoda,Brassica napus,Brassica rapa,Capsella rubella,Citrus clementina,Eutrema salsugineum,Glycine max,Glycine soja,Gossypium mexicanum,Medicago truncatula,Populus trichopocarpa,Prunus persica,Ricinus communis,Theobroma cacao,Vitis vinifera

Aufreinigung: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 UniProt: Q9LM02

Anwendungsinformationen

Applikationshinweise: 1 : 50-1 : 100 (IL), 1 : 500-1 : 1000 (WB)

Kommentare:

SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutté Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-SMT1 Antikörper (ABIN5326722)

Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).

Boutté, Frescatada-Rosa, Men, Chow, Ebine, Gustavsson, Johansson, Ueda, Moore, Jürgens, Grebe: "Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis." in: The EMBO journal, Vol. 29, Issue 3, pp. 546-58, (2010) (PubMed).

Details zu SMT1

Target: SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))

Andere Bezeichnung: Sterol methyltransferase 1 (SMT1)

Hintergrund:

Background: SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. The protein is highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD

Additional information: SMT1 is an integral membrane protein of ER (Boutte et al., 2009) while BiP is a membrane associated protein.Endogenous SMT1 is expressed at very low levels in root epidermis and root cap as compare to cortex and endodermis and therefore this can contribute to SMT1 detection problems.

Molekulargewicht: 38 kDa

Gen-ID: 831216

UniProt: Q9LM02