Sulfotyrosine Antikörper (PerCP)

Produktnummer ABIN5067502

Der Maus Monoklonal Anti-Sulfotyrosine Antikörper wurde für ELISA, ICC, IF, IHC und WB validiert. Er ist geeignet, Sulfotyrosine in Proben von zu detektieren.

Kurzübersicht für Sulfotyrosine Antikörper (PerCP) (ABIN5067502)

Target: Sulfotyrosine

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Sulfotyrosine Antikörper ist konjugiert mit PerCP

Applikation: ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Klon: 7C5

Produktdetails anti-Sulfotyrosine Antikörper (PerCP)

Spezifität: Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.

Aufreinigung: Protein G Purified

Immunogen: Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:50)
• ELISA (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN5067502 was sufficient for detection of Sulfotyrosine in 0.5 μg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu Sulfotyrosine

Target: Sulfotyrosine

Substanzklasse: Amino Acid

Hintergrund: Tyrosine sulfation is a common post-translational protein modification catalyzed by tyrosylprotein sulfotransferase (TPST) (1). Sulfotyrosines mediate interactions between hirudin and thrombin, P-selectin glycoprotein ligand-1 and P-selectin, and von Willebrand factor and factor VIII. Tyrosine sulfation of certain receptors is required for several viruses and parasites to enter their hosts.