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Sulfotyrosine Antikörper (FITC)

Der Maus Monoklonal Anti-Sulfotyrosine-Antikörper wurde für ELISA, ICC, IF, IHC und WB validiert. Er ist geeignet, Sulfotyrosine in Proben von zu detektieren.
Produktnummer ABIN5067499

Kurzübersicht für Sulfotyrosine Antikörper (FITC) (ABIN5067499)

Target

Sulfotyrosine

Wirt

  • 9
Maus

Klonalität

  • 9
Monoklonal

Konjugat

  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Sulfotyrosine Antikörper ist konjugiert mit FITC

Applikation

ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Klon

7C5
  • Spezifität

    Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.

    Aufreinigung

    Protein G Purified

    Immunogen

    Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).

    Isotyp

    IgG1
  • Applikationshinweise

    • WB (1:1000)
    • ICC/IF (1:50)
    • ELISA (1:1000)
    • IHC (1:25)
    • optimal dilutions for assays should be determined by the user.

    Kommentare

    A 1:1000 dilution of ABIN5067499 was sufficient for detection of Sulfotyrosine in 0.5 μg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Conjugated antibodies should be stored at 4°C
  • Target

    Sulfotyrosine

    Substanzklasse

    Amino Acid

    Hintergrund

    Tyrosine sulfation is a common post-translational protein modification catalyzed by tyrosylprotein sulfotransferase (TPST) (1). Sulfotyrosines mediate interactions between hirudin and thrombin, P-selectin glycoprotein ligand-1 and P-selectin, and von Willebrand factor and factor VIII. Tyrosine sulfation of certain receptors is required for several viruses and parasites to enter their hosts.
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