Dityrosine Antikörper (HRP)

Produktnummer ABIN5067464

Dieses Anti-Dityrosine-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Dityrosine in WB, ELISA, ICC und IF. Geeignet für .

Kurzübersicht für Dityrosine Antikörper (HRP) (ABIN5067464)

Target: Dityrosine (DT)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Dityrosine Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: 7D4

Produktdetails anti-Dityrosine Antikörper (HRP)

Spezifität: Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.

Aufreinigung: Protein G Purified

Immunogen: Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:50)
• ELISA (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN5067464 was sufficient for detection of dityrosine in 1 μg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu Dityrosine

Target: Dityrosine (DT)

Andere Bezeichnung: Dityrosine

Substanzklasse: Dipeptide

Hintergrund: ROS such as hydrogen peroxide (H2O2), superoxide, and hydroxyl radicals can react with both the backbone and the side chains of proteins, leading to backbone cleavage and side-chain modifications, respectively. Peroxidases, UV radiation, and hydroxyl radicals catalyze the formation of tyrosyl radicals which then react to form cross-links between proteins (1). This produces dityrosine, a metabolically stable biomarker of protein oxidation (2).