Methylglyoxal (MG) Antikörper (Biotin)

Produktnummer ABIN5067390

Produktdetails

Target: Methylglyoxal (MG)

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Spezifität: Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, MDA, 4-HHE, 4-HNE, or Crotonaldehyde modified proteins.

Aufreinigung: Protein G Purified

Immunogen: Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).

Klon: 9E7

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:50)
• FACS (1:50)
• FCM (1:50)
• ELISA (1:1000)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN5067390 was sufficient for detection of Methylglyoxal in 0.5 μg of Methylglyoxal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Antigendetails

Target: Methylglyoxal (MG)

Andere Bezeichnung: Methylglyoxal

Substanzklasse: Chemical

Hintergrund: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Methylglyoxal is an alpha-oxoaldehyde generated from lipid peroxidation that reacts with proteins to form adducts(1). It reacts with free amino acids and protein residues to generate advanced glycation endproducts (AGEs) and crosslinks DNA polymerase and substrate DNA to severely inhibit DNA replication (2).