Acrolein Antikörper (HRP)

Produktnummer ABIN5067194

Dieses Anti-Acrolein-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Acrolein in ELISA, FACS, WB, ICC, IF und IHC. Geeignet für .

Kurzübersicht für Acrolein Antikörper (HRP) (ABIN5067194)

Target: Acrolein (ACR)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Acrolein Antikörper ist konjugiert mit HRP

Applikation: ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Klon: 10A10

Produktdetails anti-Acrolein Antikörper (HRP)

Spezifität: Specific for Acrolein modified proteins. Does not detect free acrolein. Does not X-react with Crotonaldehyde, Hexanoyl Lysine, 4-HHE, 4-HNE, MDA, or Methylglyoxal modified proteins.

Aufreinigung: Protein G Purified

Immunogen: Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:50)
• FACS (1:50)
• FCM (1:50)
• ELISA (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN5067194 was sufficient for detection of Acrolein in 2 μg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu Acrolein

Target: Acrolein (ACR)

Andere Bezeichnung: Acrolein

Substanzklasse: Chemical

Hintergrund: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Acrolein is an electrophilic conjugated aldehyde that is a terminal product of lipid peroxidation. Acrolein is highly mutagenic and reacts with nucleophilic functional groups in DNA and proteins such as cysteine, histidine, and lysine residues (1).