Arginylation (N-Term) Antikörper (Atto 390)

Produktnummer ABIN5066966

Dieses Anti--Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von in WB und FACS. Geeignet für .

Kurzübersicht für Arginylation (N-Term) Antikörper (Atto 390) (ABIN5066966)

Target: Arginylation

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Atto 390

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Klon: 4A9

Produktdetails

Bindungsspezifität: N-Term

Spezifität: Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.

Aufreinigung: Protein G Purified

Immunogen: Synthetic N-terminal arginylated peptide conjugated to KLH

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• FCM (1:50)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN5066966 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Antigendetails

Target: Arginylation

Substanzklasse: Chemical

Hintergrund: Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood (1). Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress (2).