RNF216 Antikörper (N-Term)

Produktnummer ABIN501040

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch RNF216 in WB, IHC (p) und EIA. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für RNF216 Antikörper (N-Term) (ABIN501040)

Target: RNF216 (Ring Finger Protein 216 (RNF216))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNF216 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-RNF216 Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody detects RNF216 / TRIAD3.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse

Aufreinigung: Ion exchange chromatography

Immunogen: TRIAD3A antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of mouse TRIAD3A.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 0.5 to 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu RNF216

Target: RNF216 (Ring Finger Protein 216 (RNF216))

Andere Bezeichnung: RNF216

Hintergrund: Activation of NF-kappaB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2). Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR (3,4). Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase (5). TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9, this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation (5).Synonyms: E3 ubiquitin-protein ligase RNF216, RING finger protein 216, TRIAD3, Triad domain-containing protein 3, UBCE7IP1, Ubiquitin-conjugating enzyme 7-interacting protein 1, ZIN, Zinc finger protein inhibiting NF-kappa-B

Gen-ID: 54476