Radical Fringe (AA 21-120) Antikörper (AbBy Fluor® 750)

Produktnummer ABIN5009540

Der AbBy Fluor® 750-konjugierte Kaninchen Polyklonal anti-Radical Fringe Antikörper (ABIN5009540) detektiert spezifisch Radical Fringe in IF (cc) und IF (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Radical Fringe (AA 21-120) Antikörper (AbBy Fluor® 750) (ABIN5009540)

Target: Radical Fringe

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: AbBy Fluor® 750

Applikation: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails

Bindungsspezifität: AA 21-120

Homologie: Human,Mouse,Rat

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Radical Fringe

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Radical Fringe

Hintergrund:

Synonyms: 3-N-acetylglucosaminyltransferase radical fringe, Beta-1, Beta-1,3-N-acetylglucosaminyltransferase radical fringe, O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, RFNG, RFNG_HUMAN.

Background: Three mammalian fringe family members, Manic, Radical and Lunatic Fringe, have been identified as proteins related to Drosophila Fringe, a protein involved in development. Fringe proteins act upstream of the Notch signaling pathway and are involved in boundary determination during segmentation. Each mammalian Fringe displays different patterns of expression, though all are expressed in the mouse embryo as well as in many adult tissues. Radical Fringe, also known as Beta-1,3-N-acetylglucosaminyltransferase Radical Fringe, is a 331 amino acid single-pass type II membrane protein that localizes to the membrane of the Golgi apparatus. Playing a key role in the development of the limb bud, Radical Fringe transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor. Lunatic Fringe is required for normal somite segmentation and patterning and is thought to be a target of the molecular clock. Manic Fringe, also involved in somatic development, has been shown to render mouse NIH/3T3 cells tumorigenic in SCID mice.