SKIP (C-Term) Antikörper

Produktnummer ABIN500736

Dieses Anti--Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von in WB, IF und EIA. Geeignet für Human, Maus und Ratte.

Kurzübersicht für SKIP (C-Term) Antikörper (ABIN500736)

Target: SKIP

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails

Bindungsspezifität: C-Term

Spezifität: This antibody detects SKIP.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse, rat

Aufreinigung: Peptide affinity chromatography

Immunogen: SkiP antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human SkiP.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 0.5 - 1 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: SKIP

Hintergrund: TGF-beta and the bone morphogenic proteins (BMPs) are key signaling proteins that regulate numerous cellular processes such as embryonic development and tumorigenesis (1). Both signal through the Smad protein family and are negatively regulated by Ski and SnoN, two related proto-oncoproteins (2,3). Ski functions by binding to the Smad proteins activated by TGF-beta and the (BMPs) and preventing their phosphorylation, inhibiting their ability to bind DNA and activate the transcription of downstream genes (4,5). SkiP was originally identified as a Ski-interacting protein (6) and was later found to augment the signals induced by TGF-beta but inhibit transcription induced by BMP-2 (7) in C2C12 cells, suggesting that SkiP is a key player in the signaling cascades inititated by TGF-beta and the BMP protein family.Synonyms: NCoA-62, Nuclear protein SkiP, Nuclear receptor coactivator NCoA-62, SKIIP, SNW domain-containing protein 1, SNW1, Ski-interacting protein

Gen-ID: 22938

NCBI Accession: NP_036377

UniProt: Q13573