MED28 Antikörper (Center)

Produktnummer ABIN500268

Dieses Anti-MED28-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MED28 in WB, IHC (p) und EIA. Geeignet für Human und Maus.

Kurzübersicht für MED28 Antikörper (Center) (ABIN500268)

Target: MED28 (Mediator Complex Subunit 28 (MED28))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED28 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-MED28 Antikörper

Bindungsspezifität: Center

Spezifität: This antibody detects MED28.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse

Aufreinigung: Peptide affinity chromatography

Immunogen: MED28 antibody was raised against a 17 amino acid peptide from near the center of human MED28.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu MED28

Target: MED28 (Mediator Complex Subunit 28 (MED28))

Andere Bezeichnung: MED28

Hintergrund: The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED28, also known as Magicin. MED28 is expressed in many cell lines and tissues. It has been shown that a down-regulation of MED28 expression in NIH3T3 cells results in a significant induction of several genes associated with smooth muscle cell differentiation and conversely its over-expression represses expression of SMC genes. MED28 can also form a ternary complex with Grb2 and Merlin, the neurofibromatosis 2 tumor suppressor protein, indicating that MED28 may play a role in Merlin's tumor suppressive activity. MED28 has also been recently identified as an HIV dependency factor (HDF), suggesting that MED28 may be an important drug target in HIV treatment. At least two isoforms of MED28 are known to exist.Synonyms: EG1, Endothelial-derived protein 1, FKSG20, Mediator complex subunit 28, Mediator of RNA polymerase II transcription subunit 28, Merlin and Grb2-interacting cytoskeletal protein, Tumor angiogenesis marker EG-1

Gen-ID: 80306

NCBI Accession: NP_079481

UniProt: Q9H204

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation