MADD Antikörper (C-Term)

Produktnummer ABIN500216

Der Kaninchen Polyklonal anti-MADD Antikörper wird verwendet zum Nachweis von MADD in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IF und EIA.

Kurzübersicht für MADD Antikörper (C-Term) (ABIN500216)

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MADD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-MADD Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody detects MADD at C-term.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse, rat

Aufreinigung: DEAE purified

Immunogen: MADD antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human MADD.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 1: 250 - 1: 500 dilution, 200 to 220 kDa bands should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu MADD

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Andere Bezeichnung: MADD

Hintergrund: MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1) associated protein though their death domains. Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98 % identity with DENN (for differentially expressed in neoplastic vs. normal cells), which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greater than 94 % overall identity to a GDP/GTP exchange protein Rab3-GEP. MADD is 87 % identical to KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signaling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.Synonyms: DENN, IG20, MAP kinase-activating death domain protein, RAB3GEP, Rab3 GDP/GTP exchange factor

Gen-ID: 8567

UniProt: Q8WXG6

Pathways: Caspase Kaskade in der Apoptose