GOLPH3 Antikörper (N-Term)

Produktnummer ABIN499904

Der Kaninchen Polyklonal Anti-GOLPH3-Antikörper wurde für WB und EIA validiert. Er ist geeignet, GOLPH3 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für GOLPH3 Antikörper (N-Term) (ABIN499904)

Target: GOLPH3 (Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GOLPH3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-GOLPH3 Antikörper

Bindungsspezifität: N-Term

Aufreinigung: Affinity chromatography purified via peptide column

Immunogen: GOLPH3 antibody was raised against a 14 amino acid peptide from near the amino terminus of human GOLPH3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu GOLPH3

Target: GOLPH3 (Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3))

Andere Bezeichnung: Golgi Phosphoprotein 3 / GOLPH3

Hintergrund: GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.Synonyms: Coat protein GPP34, GPP34, MIDAS, Mitochondrial DNA absence factor

Gen-ID: 64083

UniProt: Q9H4A6