CNOT4 Antikörper (N-Term)

Produktnummer ABIN499646

Produktdetails anti-CNOT4 Antikörper

Target: CNOT4 (CCR4-NOT Transcription Complex Subunit 4 (CNOT4))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CNOT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody reacts to CNOT4.

Aufreinigung: Affinity chromatography purified via peptide column

Immunogen: CNOT4 antibody was raised against a 19 amino acid peptide near the amino terminus of the human CNOT4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: CNOT4 antibody can be used for detection of CNOT4 at 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu CNOT4

Target: CNOT4 (CCR4-NOT Transcription Complex Subunit 4 (CNOT4))

Andere Bezeichnung: CNOT4 (CNOT4 Produkte)

Hintergrund: CNOT4 is a component of the CCR4-NOT transcription complex, a complex that is implicated in the repression of RNA polymerase II transcription. In the CCR4-NOT complex, CNOT4 acts as an E3 ubiquitin-protein ligase and interacts with a subset of E2 ubiquitin-conjugating enzymes through a unique C4C4 RING domain. This E3 ligase activity was shown to be dependent on the selective and specific interaction with the ubiquitin conjugating enzyme UbcH5B. In yeast, mutations in CNOT4 that prevented its interaction with the UbcH5B homolog UBC4 caused increased sensitivity to hydroxyurea, heat shock, and hygromycin B, suggesting that CNOT4 and UbcH5B are involved in stress response in vivo. Multiple isoforms of CNOT4 are known to exist.Synonyms: CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, E3 ubiquitin-protein ligase CNOT4, NOT4, NOT4Hp, Potential transcriptional repressor NOT4Hp

Gen-ID: 4850

NCBI Accession: NP_037448

UniProt: O95628