CDC27 Antikörper (C-Term)

Produktnummer ABIN499295

Der Kaninchen Polyklonal anti-CDC27 Antikörper wird verwendet zum Nachweis von CDC27 in Proben von Human, Ratte und Maus. Er wurde validiert für WB und EIA.

Kurzübersicht für CDC27 Antikörper (C-Term) (ABIN499295)

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDC27 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CDC27 Antikörper

Bindungsspezifität: C-Term

Aufreinigung: Affinity chromatography purified via peptide column

Immunogen: APC3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human APC3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: APC3 antibody can be used for detection of APC3 at 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu CDC27

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Andere Bezeichnung: CDC27

Hintergrund: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC3, also known as CDC27, a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), is a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. APC3 contains a tetratricopeptide repeat (TPR) region and interacts with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1.Synonyms: CDC27Hs, Cell division cycle protein 27 homolog, D0S1430E, D17S978E, H-NUC

Gen-ID: 996

NCBI Accession: NP_001247

UniProt: P30260