AFAP1L2 Antikörper (C-Term)

Produktnummer ABIN499229

Der Kaninchen Polyklonal Anti-AFAP1L2-Antikörper wurde für WB, IHC (p) und EIA validiert. Er ist geeignet, AFAP1L2 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für AFAP1L2 Antikörper (C-Term) (ABIN499229)

Target: AFAP1L2 (Actin Filament Associated Protein 1-Like 2 (AFAP1L2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AFAP1L2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-AFAP1L2 Antikörper

Bindungsspezifität: C-Term

Spezifität: This anitbody reacts to AFAP1L2.

Aufreinigung: Affinity chromatography purified via peptide column

Immunogen: AFAP1L2 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human AFAP1L2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: AFAP1L2 antibody can be used for detection of AFAP1L2 at 1 - 2 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu AFAP1L2

Target: AFAP1L2 (Actin Filament Associated Protein 1-Like 2 (AFAP1L2))

Andere Bezeichnung: AFAP1L2

Hintergrund: AFAP1L2, also known as XB130, is structurally similar to actin-filament-associated protein (AFAP), containing several SH2- and SH3-binding motifs, two pleckstrin homology domains, a coiled-coil region, and many potential phosphorylation sites. It interacts with and is phosphorylated by c-Src tyrosine kinase. Suppression of AFAP1L2 via siRNA reduced Src activity, IL-8 production, EGF-induced phosphorylation of Akt and GSK3b, and altered cell cycles in human lung epithelial cells suggesting that AFAP1L2 plays a role as an adaptor in the regulation of Src signal transduction and multiple cellular functions. Recent experiments have shown that AFAP1L2 is highly expressed in thyroid and is the substrate RET/PTC kinase, a thyroid-specific kinase that plays a pathogenic role in papillary thyroid cancer. Down-regulation of AFAP1L2 in these cancer cells reduced Akt activity, inhibiting cell-cycle progression and cancer cell survival in suspension, indicating that AFAP1L2 may be a valuable target in thyroid cancer therapy. At least four isoforms of AFAP1L2 are known to exist.Synonyms: AFAP1-like protein 2, Actin filament-associated protein 1-like 2, KIAA1914, XB130

Gen-ID: 84632

NCBI Accession: NP_001001936

UniProt: Q8N4X5

Pathways: EGFR Signaling Pathway