Aconitase 1 Antikörper (Internal Region)

Produktnummer ABIN499173

Der Ziege Polyklonal anti-Aconitase 1 Antikörper (ABIN499173) detektiert spezifisch Aconitase 1 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Rind (Kuh).

Kurzübersicht für Aconitase 1 Antikörper (Internal Region) (ABIN499173)

Target: Aconitase 1 (ACO1)

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Aconitase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Aconitase 1 Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-QVDFNRRADS LQKNQ

Spezifität: This antibody detects ACO1 / IREB1 (Internal).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_002188.1 Genename: ACO1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1: 8000 (limit dilution). Western blot: 0.1-0.3 μg/mL. Approx 100 kDa band observed in Human Liver lysates(calculated MW of 98.0 kDa according to NP_002188.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for one week or (in aliquots) at -20 °C for longer.

Details zu Aconitase 1

Target: Aconitase 1 (ACO1)

Andere Bezeichnung: ACO1 / IREB1

Hintergrund: Aconitase 1 (ACO1) also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IRES). IREs are translational regulatory sequences found in the 5'-UTR of ferritin, and in the 3'-UTR of transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis by regulating ferritin mRNA translation and TFRC mRNA stability.Synonyms: Aconitase, Citrate hydro-lyase, Cytoplasmic aconitate hydratase, Ferritin repressor protein, IRP1, Iron regulatory protein 1, Iron-responsive element-binding protein 1

Gen-ID: 48

NCBI Accession: NP_002188

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis