CST8 Antikörper (Internal Region)

Produktnummer ABIN499119

Dieser Anti-CST8-Antikörper ist ein Ziege-Polyklonal-Antikörper zum Nachweis von CST8 in WB und EIA. Geeignet für Human.

Kurzübersicht für CST8 Antikörper (Internal Region) (ABIN499119)

Target: CST8 (Cystatin 8 (CST8))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CST8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CST8 Antikörper

Bindungsspezifität: AA 100-112, Internal Region

Sequenz: STNEICAIQE NSK

Spezifität: This antibody recognizes Cystatin 8 (aa 100-112)

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_005483.1.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/16000. Western blot: 1-3 μg/mL. In transfected HEK293 transiently expressing Human CST8 (MYCand DYKDDDDK tagged), a band of approx.23 kDa is observed. No bands are observed inmock-transfected HEK293 and the same band is observed using anti-myc tag antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CST8

Target: CST8 (Cystatin 8 (CST8))

Andere Bezeichnung: Cystatin 8

Hintergrund: The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. CST8 performs a specialized role during sperm development and maturation.Synonyms: CRES, CST8, Cystatin-related epididymal spermatogenic protein

Gen-ID: 10047

NCBI Accession: NP_005483

UniProt: O60676