HLA-DOA Antikörper

Produktnummer ABIN498962

Der Kaninchen Polyklonal anti-HLA-DOA Antikörper wird verwendet zum Nachweis von HLA-DOA in Proben von Human. Er wurde validiert für WB und IHC (p).

Kurzübersicht für HLA-DOA Antikörper (ABIN498962)

Target: HLA-DOA (HLA Class II DO alpha (HLA-DOA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HLA-DOA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HLA-DOA Antikörper

Spezifität: This antibody detects endogenous levels of HLADOalpha protein. (region surrounding Val111)

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu HLA-DOA

Target: HLA-DOA (HLA Class II DO alpha (HLA-DOA))

Andere Bezeichnung: HLA Class II DO alpha / HLA-DOA

Hintergrund: Reacts with HLA-DO, a heterodimer formed by DNalpha and DObeta subunits in B cells. DNalpha and DObeta are the products of the non-classical class II genes, HLA-DNA and HLA-DOB, respectively. DO forms tight complexes with DM in the endoplasmic reticulum and is thereby sorted to lysosomal vesicles during antigen processing and presentation. It is tightly associated with DM and it is selectively expressed on antigenpresenting cells, such as B cells, dendritic cells and thymic epithelial cells. DO can enhance the efficiency of peptide loading and has been found to stabilize DM at low pH , preserving its chaperon activity. DO-DM complexes are more efficient than DM in protecting empty DR molecules. Reports describe DO as a co-chaperone of DM. This antibody has also been reported to be suitable for western blot and immunoprecipitation.Synonyms: HLA class II histocompatibility antigen DO alpha chain, HLA-DNA, HLA-DZA, MHC DN-alpha, MHC DZ alpha, MHC class II antigen DOA

Molekulargewicht: approx. 34 kDa

Gen-ID: 3111

NCBI Accession: NP_002110

UniProt: P06340

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response