GATA1 Antikörper

Produktnummer ABIN498932

Der Kaninchen Polyklonal anti-GATA1 Antikörper wird verwendet zum Nachweis von GATA1 in Proben von Human, Maus und Ratte. Er wurde validiert für IHC (p).

Kurzübersicht für GATA1 Antikörper (ABIN498932)

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GATA1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GATA1 Antikörper

Spezifität: This antibody detects endogenous levels of GATA-1 protein. (region surrounding Thr148)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu GATA1

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Andere Bezeichnung: GATA1

Hintergrund: Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. GATA-4 and GATA-6 are found in heart, pancreas and ovary, lung and liver tissues exhibit GATA-6, but not GATA-4, expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart.Synonyms: ERYF1, Eryf1, Erythroid transcription factor, GATA-1, GATA-binding factor 1, GF-1, GF1, NF-E1 DNA-binding protein

Molekulargewicht: approx. 43 kDa

Gen-ID: 2623

NCBI Accession: NP_002040

UniProt: P15976

Pathways: Cellular Response to Molecule of Bacterial Origin