Merlin Antikörper (pSer518)

Produktnummer ABIN498767

Der Kaninchen Polyklonal anti-Merlin Antikörper wird verwendet zum Nachweis von Merlin in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IF.

Kurzübersicht für Merlin Antikörper (pSer518) (ABIN498767)

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Merlin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-Merlin Antikörper

Bindungsspezifität: pSer518

Spezifität: This antibody detects endogenous levels of p-NF2 protein only when phosphorylated at Ser518.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Merlin

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Andere Bezeichnung: Merlin

Hintergrund: Neurofibromatosis type 2 (NF2) is a dominantly inherited disorder characterized by the occurance of bilateral vestibular schwannomas and other central nervous system tumors, including multiple meningiomas. NF2 occurs in about 1 of 40,000 live births. The NF2 gene is highly penetrant, NF2-affected individuals have a 95 % chance of developing bilateral vestibular schwannomas. NF2 is distinct from NF1, which is characterized by an incidence of 1 in 4000, maps to chromosome 17 and encodes a protein designated neurofibromin, which is a large protein with a GAP domain. Genetic linkage studies of both sporadic and familial tumors suggest that NF2 is caused by inactivation of a tumor suppressor gene that maps on chromosome 22q12 and encodes a 595 amino acid protein whose function appears to be mediated by interaction with the cytoskeleton.Synonyms: Moesin-ezrin-radixin-like protein, NF2, Neurofibromin-2, SCH, Schwannomerlin, Schwannomerlin, Schwannomin, Schwannomin

Molekulargewicht: approx. 70 kDa

Gen-ID: 4771

NCBI Accession: NP_000259

UniProt: P35240

Pathways: Cell-Cell Junction Organization