Pepsin Antikörper

Produktnummer ABIN498687

Der Kaninchen Polyklonal anti-Pepsin Antikörper (ABIN498687) detektiert spezifisch Pepsin in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Pepsin Antikörper (ABIN498687)

Target: Pepsin

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Pepsin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Pepsin Antikörper

Spezifität: This antibody detects endogenous levels of Pepsin protein. (region surrounding Met307)

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Pepsin

Target: Pepsin

Hintergrund: Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity ( pH 1-3) for Pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity, although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. Pepsin A is a member of the subfamily A1 within the Pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates. Pepsin A is inhibited by ovUS-1, a uterine Serpin.Synonyms: PGA, PGA3, PGA4, PGA5, Pepsin A, Pepsinogen A, pepsinogen 3 group I, pepsinogen 4 group I, pepsinogen 5 group I

Molekulargewicht: approx. 42 kDa

Gen-ID: 5222

NCBI Accession: NP_001073276

UniProt: P00790