PKC theta Antikörper

Produktnummer ABIN498658

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PKC theta in WB, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.

Kurzübersicht für PKC theta Antikörper (ABIN498658)

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKC theta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PKC theta Antikörper

Spezifität: This antibody detects endogenous levels of PKC theta protein. (region surrounding asn670)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PKC theta

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Andere Bezeichnung: PRKCQ / PRKCT

Hintergrund: Members of the protein kinase C (PKC) family play a key regulatory role in variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, epsilon , zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilonare independent of Ca++. On the other hand, nPKC and epsilon, as well as all of the cPKC members, possess phorbol esterbinding activities and kinase activities.Synonyms: PKC theta, PKC-theta, Protein kinase C theta type, Protein kinase C theta type, nPKC-theta

Molekulargewicht: approx. 80 kDa

Gen-ID: 5588

NCBI Accession: NP_006248

UniProt: Q04759

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling