MARCKS Antikörper

Produktnummer ABIN498653

Produktdetails anti-MARCKS Antikörper

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MARCKS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of MARCKS protein. (region surrounding Arg157)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Andere Bezeichnung: MARCKS (MARCKS Produkte)

Synonyme: 80K-L antikoerper, MACS antikoerper, PKCSL antikoerper, PRKCSL antikoerper, KINC antikoerper, Macs antikoerper, marcks antikoerper, MGC89127 antikoerper, MARCKS antikoerper, macs antikoerper, wu:fb55c06 antikoerper, wu:fb92d04 antikoerper, wu:fc19b04 antikoerper, wu:fc44a10 antikoerper, zgc:110235 antikoerper, fc34g03 antikoerper, fc38c06 antikoerper, wu:fc34g03 antikoerper, wu:fc38c06 antikoerper, zgc:109978 antikoerper, myristoylated alanine rich protein kinase C substrate antikoerper, myristoylated alanine-rich protein kinase C substrate antikoerper, methyl binding domain106 antikoerper, myristoylated alanine-rich protein kinase C substrate b antikoerper, myristoylated alanine-rich protein kinase C substrate S homeolog antikoerper, myristoylated alanine-rich protein kinase C substrate a antikoerper, MARCKS antikoerper, Marcks antikoerper, marcks antikoerper, mbd106 antikoerper, marcksb antikoerper, marcks.S antikoerper, marcksa antikoerper

Hintergrund: Myristoylated alanine-rich protein kinase C substrate (MARCKS), also designated 80K or 80K-L, has been identified as a major cellular substrate for protein kinase C. Human MARCKS is a 332 amino acid protein with a calculated molecular weight of 31.534 kDa, however, it has been shown to run at 80-87 kDa on Western blot. The plasma membrane bound protein dissociates from the membrane upon phosphorylation by various PKC isoforms. In NIH/3T3 fibroblasts, PKC alpha and PKC epsilon, but not PKC delta, are responsible for MARCKS phosphorylation. MARCKS has been found to bind calmodulin, Actin and Synapsin and is a filamentous (F) Actin crosslinking protein.Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL

Molekulargewicht: approx. 32, 75, 87 kDa

Gen-ID: 4082

NCBI Accession: NP_002347

UniProt: P29966