DDX3X Antikörper

Produktnummer ABIN498119

Der Kaninchen Polyklonal anti-DDX3X Antikörper wird verwendet zum Nachweis von DDX3X in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC (p).

Kurzübersicht für DDX3X Antikörper (ABIN498119)

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDX3X Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-DDX3X Antikörper

Spezifität: This antibody detects endogenous levels of DDX3 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography using epitope-specific immunogen

Reinheit: > 95 % (by SDS-PAGE)

Anwendungsinformationen

Applikationshinweise: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu DDX3X

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Andere Bezeichnung: DDX3X

Hintergrund: DDX3 contains all of the motifs of the DEAD box family of RNA helicases, including the Asp/Glu/Ala/Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It is also involved in cell growth control and is deregulated in hepatitis virus associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.Synonyms: ATP-dependent RNA helicase DDX3X, DBX, DDX3, DEAD box X isoform, DEAD box protein 3 X-chromosomal, DEAD3, Embryonic RNA helicase, HLP2, Helicase-like protein 2

Molekulargewicht: approx. 75 kDa

Gen-ID: 1654

NCBI Accession: NP_001347

UniProt: O00571

Pathways: Ribonucleoprotein Complex Subunit Organization, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Ribosome Assembly